Revista de Ciencias Agrícolas <p align="justify">The Revista de Ciencias Agrícolas is a recognized means of divulgation and distribution of scientific and technological research nationwide and abroad, biannually publishes original articles written in english, on topics related to Agricultural Sciences, which present in detail results of research in agriculture, forestry, agricultural biotechnology and food which are subject to the assessment process by nationals and foreign peers, and to review and approval by the Editorial Committee of the magazine.</p> <p><strong>DOI:</strong>&nbsp;<a href="" target="_blank" rel="noopener"></a></p> <p>&nbsp;</p> Universidad de Nariño en-US Revista de Ciencias Agrícolas 0120-0135 Effect of Storage Conditions on Physicochemical Characteristics and Phenolic Compounds of Eggplant (Solanum melongena L.) <p>High demand for fresh fruit and vegetables requires the implementation of techniques to extend the shelf life of these products, which are highly perishable. A widely used method for this purpose is the refrigeration; however, it is known that the most of vegetables preserved in this way evidence chilling injury signs during storage. Eggplants cv C029 recently harvested were stored at 13°C packed into low density polyethylene bags either with perforations (PPB), vacuum packaged (VPB) or unpacked during 5, 10, 15 and 20 days in order to evaluate the effect of chilling on physicochemical characteristics and visual appearance. Unpacked Eggplants presented remarkable chilling injury symptoms, losing its commercial quality before 10d of storage. Fruit stored in VPB showed discoloration affecting visual appearance but maintains the phenolic compounds and avoided pulp and seeds browning. Eggplants packed in PPB showed the best results in this work, preserving overall characteristics during 15d.</p> José Andrés Barragán López Arlet Patricia Franco Peñata Jessica López Pastén Carmen Elena Pérez Cervera ##submission.copyrightStatement## 2019-12-19 2019-12-19 36 2 Tangential Microfiltration of blackberry (Rubus adenotrichus Schltdl.) juice sweetened with stevia (Stevia rebaudiana Bertoni) <p>The modern consumer values those components that can promote their well-being and help them reduce the risk of diseases the use of steviolglucosides as a sweetener in the formulation of foods is promising. The study evaluated the tangential microfiltration for clarification and conservation of blackberry juice sweetened with dried leaves of stevia. Two ceramic membranes were evaluated. The tests were performed at 35°C in concentration mode at a constant transmembrane pressure and tangential velocity. The formulation of the juice was defined through sensory evaluation tests.&nbsp;&nbsp; Volume, flow permeate, temperature and pressure input and output were registered. The results of the variables analyzed in the process, as well as the physicochemical characterization of clarified juice, helped define the operating conditions for draw two formulations of microfiltrated juice, sweetened with stevia or sucrose, and thus evaluate the effectiveness of microfiltration as conservation treatment. Aciduric microorganisms were quantified; molds and yeasts were carried out in the generated pasteurized samples stored at five different temperatures (5, 20, 30, 37 and 45°C).&nbsp; The recovery permeate juice obtained was 84.62%.&nbsp; The permeate Flow (Jp), exhibited higher values ​​at 40L/hm<sup>2</sup>. Also, the concentration of stevioside and rebaudioside A in the retentate increased with advancing ultrafiltration.&nbsp; A significant variation in color, turbidity and soluble solids, was observed, unlike the pH.&nbsp;&nbsp; Also, its chemical and microbiological stability is confirmed that allow you to preserve quality during 27 days of storage.</p> César González Torrivilla Fabrice Vaillant Barka María Soledad Tapia González ##submission.copyrightStatement## 2020-02-19 2020-02-19 36 2 Soil properties and storage of organic carbon in the soil uses pasture and forest <p>Soil is probably one of the natural resources most vulnerable to climate change. Composes most of the carbon stock in terrestrial ecosystems. The aims of this study were: to characterize the use of pasture land and native forest in two sites in El Oro province (Ecuador), based on their physical, chemical and biological properties, and to compare the Soil Organic Carbon (SOC) stock at three depths. For this purpose, plots were established in <em>Brachiaria sp</em> pastures and native forests in the coastal area of Machala, Santa Inés site (SI) and in the mountains of Chilla, Cune site (CN). Soil sampling was done at three depths (C1=0-10cm; C2=10-20cm; C3= 20-3 cm). The SI soils showed significance (p-value&lt;0,05) among the stored SOC layers. The highest stored SOC value was for SI forest (C1, 36,30megagrams-Mg C ha<sup>-1</sup>), in an alkaline pH and clay loam texture. In CN soils, stored COS revealed no significance between layers and values ranged from 27,64Mg ha<sup>-1</sup>(C1, CN<sub>forest</sub>) to 35,01 Mg C ha<sup>-1</sup> (C3, CN<sub>pastur</sub>e), in an acidic pH, sandy loam texture and high levels of Fe (761,71-938,34 mg kg<sup>-1</sup>). Soil properties play an important role in stored SOC levels; therefore, sequestration of SOC must be considered to develop optimal pasture management.</p> Salomón Alejandro Barrezueta Unda Kelvin Adrián Velepucha Cuenca Luis Hurtado Flores Edwin Edison Jaramillo Aguilar ##submission.copyrightStatement## 2020-02-19 2020-02-19 36 2 Genetic diversity and geographical genetic diversity in Colombian accessions of Lippia alba (Mill.) N.E. Brown <p>Bushy matgrass (<em>Lippia alba</em>, Verbenaceae) is a promising plant genetic resource, by their active compounds. The present document studied the molecular genetic diversity and spatial genetic structure on two contrasting populations of <em>L. alba</em> in Colombia. Eight RAM, evaluated total DNA of 59 accessions of non-cultivated plants collected in two Colombia regions, Chicamocha and Sumapaz. The expected average heterozygosity (or average heterozygosity genetic diversity of Nei) for the sample had low value (<em>0.0≤H<sub>e</sub>=0.2467≤0.5</em>). The values of molecular diversity indicated values in the range of 0.1219 to 0.3425 for seven RAM. The frequency of variants is based on effective number of alleles [Ae] and expected heterozygosity [<em>H<sub>e</sub></em>], genetic diversity by locus (<em>hj=1-p<sup>2</sup>-q<sup>2</sup></em>) had maximum values (near 0.5) in the primers ACA-AG-CGA-CEC. RAM suitably analyzed <em>Lippia alba</em> as endemic genetic resource. A DNA bank composed of 59 Colombian accessions from <em>Lippia alba</em> was set up. The analysis of the spatial global structure shows that the subpopulation Sumapaz is structured, whilst the subpopulation Chicamocha, is in structuring process. The results suggest in all cases the need for implementing: a) exchange of gene-seed, (b) gene banks of minimum, with a maximum genetic variability, c) induce genetic diversity.</p> <p><strong><em>&nbsp;</em></strong></p> José Omar Cardona Montoya ##submission.copyrightStatement## 2020-02-19 2020-02-19 36 2 Adaptation of the land suitability evaluation system for highlands vineyards in Santa Catarina State, Brazil <p>Adequate information from the agricultural suitability of the land for viticulture is of great importance to expansion of activity in wine producing regions, such as in the state of Santa Catarina State, Brazil. In this context, this study aimed to adapt the system for evaluating agricultural potential of the land (SAAAT) favored by Ramalho Filho e Beek (1995) for the culture of the vine produced in altitude region in the state of Santa Catarina. To this end the SAAAT was modified from the requirements and form of crop management, and proposed the merger, removal, adjustment and parameters used in SAAAT updates. The limiting factors considered were: a) Topography; b) Fertility; c) Excess water or oxygen deficiency; d) susceptibility to erosion; e) Impediment to mechanization; and f) Soil effective depth. These parameters resulted bibliography data collection and were related to them five degrees of limitation: null; light; medium; forte and very strong. Agricultural suitability classes (Good, Regular, Restricted and unfit) resulted from the interaction of agricultural conditions and management level of winemakers and in the end, was adapted the fitness guide frame. The adaptation of SAAAT for the vine to the specific altitude conditions in the state of Santa Catarina incorporates and adjusts sites relevant to the grape production system. It is becoming important tool for decision making in the implementation of new vineyards, contributing to possible expansion of this activity in the region.</p> Denilson Dortzbach Arcangelo Loss Marcos Gervasio Pereira Ludmila Nascimento Machado Lúcia Helena Cunha dos Anjos ##submission.copyrightStatement## 2020-02-19 2020-02-19 36 2 Activity of nitrifying bacteria and productivity of crops under the use of green manure <p>During the recycling of soil nitrogen, it is necessary to register the activity of nitrifying bacteria in nitrification. This process in tropical conditions is negatively affected by extreme variations in temperature, humidity, pH and organic matter during agronomic practices in agroecosystems. Population changes of nitrifying bacteria in corn and soybean crops were evaluated under the use and management of green manure (GM). It was established as GM <em>Mucuna pruriens</em> var. <em>utilis</em> (CIAT No. 9349) - corn var. ICA 305. Ninety days later it was harvested and the residues were incorporated as LF or disposed on the soil surface as organic mulch (OM), in parallel the native weed <em>Rottboellia cochinchinensis</em> L. was managed as fallow (F). Then, corn-soybean crops were sown in an intercalated and monoculture manner, each system was subject to organic fertilization (OF), Chemical fertilization (CF) or no fertilization (NF). 11 treatments were structured under the design of Random Complete Blocks with 3<sup>2</sup> + 2 factorial arrangements with three repetitions. In the stage of blossoming and grain filling of the crops, the variables analyzed were; oxidizing bacteria of ammonium nitrate, volumetric humidity, temperature and yield. The populations of AOB and the production of NO<sub>3</sub><sup>-</sup> did not vary significantly between treatments, but it did occur with soil temperature and humidity. The use of GM / OM promoted yields similar to those obtained with CF in corn, while in soybeans, when OM was added, yields exceeded CF.</p> Breno Sosa R. Yuly García V. Glauco Sosa R. ##submission.copyrightStatement## 2020-02-19 2020-02-19 36 2 Integrated management of Colletotrichum gloesporioides in yellow passion fruit (Passiflora edulis f. flavicarpa Degener) <p>Anthracnose (<em>Colletotrichum gloeosporioides</em>) is one of the most limiting diseases of yellow passion fruit, reducing crop production by more than 80%. This research aimed to assess anthracnose management strategies on yellow passion fruit crop sanitation and yield. We assessed the effect of alternating fungicide applications (//) every 15 days with foliar nutrients on the severity of anthracnose and crop yield in a commercial crop in San Isidro district (Montería), located at coordinates 8°44'52.7'' N 75°52'53.2''W and 75m a.s.l. We conducted this research in two experiments; in the first, we assessed the response of the disease index (DI) after the application of the following treatments: T1=Manazeb (Mancozeb) // Potassium phosphite (Foscrop), T2=Mancozeb // Potassium (Terramin potassium), T3=Mancozeb // Calcium (Terramin calcium), T4=<em>Trichoderma</em> spp. (Fitotripen) // Copper oxychloride, T5=Azoxystrobin (Amistar) // Mancozeb, and T6=Control sprayed with water. In the second experiment, we selected the best treatments, as follow: T1 = Amistar // Mancozeb; T2 = Terramin potassium // Mancozeb, and T3 = Control. We evaluated DI, percent of affected fruits, number of fruits/ha, and yield (Kg/ha). The results from the first experiment showed that T1 and T5 controlled anthracnose, reducing the levels of severity by 39.69% compared to the control (T6). In the second experiment, T1 decreased the levels of foliage severity by 29.04% and increased the number of fruits/ha and yield by 200% compared to the control. Overall, the fungicide application of Azoxistrobin alternating with Manazeb constitutes an effective alternative to control anthracnose in yellow passion fruit cultivation.</p> Rodrigo Orlando Campo-Arana Naudith Urango-Esquivel Dairo Pérez-Polo ##submission.copyrightStatement## 2020-02-19 2020-02-19 36 2