Molecular characterizacion of Trichoderma spp. in pea Pisum sativum L.

Authors

  • Oscar Eduardo Checa C. Universidad de Nariño http://orcid.org/0000-0002-6929-7717
  • Jessica Andrea Descance V. Universidad de Nariño
  • Maribel Xiomara Toro C. Universidad de Nariño
  • Sandra Lorena Álvarez
  • Claudia Salazar G. Universidad de Nariño

DOI:

https://doi.org/10.22267/rcia.153202.8

Keywords:

Genetic diversity, beneficial fungus, RAPDs.

Abstract

The genetic variability of Trichoderma spp isolates was determined through RAPD markers. A high polymorphism was found with primers OPB 01, OPC 01, OPA 03, OPD 05, and OPA 01. The observed molecular variation was low (26%) among Trichoderma spp populations, which were identified by their geographical origin as Ipiales, Pupiales, and Gualmatán. In contrast, variation within populations was high, exceeding 70%. The Trichoderma spp. species showed no relationship with their geographical origin. The isolates were grouped based on Jaccard’s genetic diversity. The range of genetic distance between the different isolates was between 0.37 and 1.42. The most frequent species was T. harzianum, which showed a high variation in all clusters.

Downloads

Download data is not yet available.

References

ALABOUVETTE, C.; STEINBERG, C. 2006. The soil as a reservoir for antagonists to plant diseases. pp. 123-144. In: Eilenberg, J. and Hokkanen, H.M.T., An Ecological and Societal Approach to Biological Control, Springer, Netherlands.

BISSETT, J. 1991. A revision of the genus Trichoderma. II. Infrageneric classification. Canadian Journal of Botany. 69(11): 2357-2372.

CARLILE, M.; WATKINSON, S. 1996. The fungi. First edition. N.Y. Academic Press. London. 608 p.

CHAVERRI, P.; CASTLEBURY, L.; OVERTON, B.; SAMUELS, G. 2003. Hypocrea/Trichoderma: species with conidiophore elongations and green conidia. Mycologia. 95(6): 1100-1140.

GONZÁLEZ, S, J.; MARURI, G.; GONZÁLEZ, A. 2005. Evaluación de diferentes concentraciones de Trichoderma contra Fusarium oxysporum agente causal de la pudrición de plántulas de papaya (Carica papaya L.) en Tuxpan, Veracruz, México. Revista UDO Agrícola. 5(1): 45-47.

GRIFFITH, G.; SHAW, D. 1998. Polymorphisms in Phytophthora infestans: Four Mitochondrial Haplotypes Are Detected after PCR Amplification of ADN from Pure Cultures or from Host Lesions. Applied and Environmental Microbiology. 64(10): 4007-4014.

HARMAN, G. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopathology. 96: 190-194.

KREDICS, L.; ANTAL, Z.; DÓCZI, I.; MANCZINGER, L.; KEVEI F.; NAGY. 2003. Clinical importance of the ge- nus Trichoderma. Acta Microbiologica et Inmunologica Hungarica. 50(2): 105-117.

KREDICS, L.; HATVANI, L.; ANTAL, Z.; MANCZINGE, L.; DRUZHININA, I.; KUBICEK, C.; SZEKERES, A.; NAGY, A.; VÁGVÖLGYI, C.; NAGY, E. 2006. Green mould disease of oyster mushroom in Hungary and Transylvania. Acta Microbiologica Immunologica et Hungarica. 5(3): 306-307

KULLNIG, C.; KRUPICA, T.; WOO, S.; MACH, R.; REY, M.; BENITEZ, T.; LORITO, M.; KUBICEK. C. P. 2001. Confusion abounds over identities of Trichoderma biocontrol isolates. Mycological Research. 10(5): 769 - 772.

LARDNER, P.; JHNSTON, K.; PLUMER.; PERSON. 1999. Morphological and molecular analysis of Colleto- trichumacutatum sensulato. Mycological Research. 103(3):275 - 285.

LUNA, M.; FLORES, M.; PONCE, N. 2003. Caracterización molecular de aislados de Sclerotium cepivorum mediante análisis del polimorfismo de los fragmentos amplificados al azar. Elementos. 49: 53 - 59.

REY, M.; DELGADO, J.; RINCÓN, A.; LIMÓN, M.; BENÍTEZ, T. 2000. Mejora de cepas de Trichoderma para su empleo como biofungicidas. Revista Iberoamericana de Micología. 17: 531 - 536.

SAMUELS, G. 2006. Trichoderma: systematic, the sexual state, and ecology. Phytopathology. 96: 195 - 206.

SÁNCHEZ, V.; REBOLLEDO, O.; PICASO, R.; CÁRDENAS, E.; CÓRDOVA, J.; GONZÁLEZ, O.; SAMUELS, G. 2008. In vitro antagonism of Thielaviopsisparadoxa by Trichoderma longibrachiatum. Mycopathologia. 163: 49 - 58.

VERMA, M.; BRAR, S.; TYAG, R.; SURAMPALLI, R.; VALERO, J. 2007. Review: Antagonistic fungi, Trichoderma spp.: Panoply of biological control. Biochemical Engineering Journal. 3(7): 1 20.

WILLIAMS, G.; KUBELIK, R.; LIVAK, J.; RAFALSKI, J.; ANTONI,; TINGEY, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research. 18(22): 6531.

WOO, S.; SCALA, F.; RUOCCO, M.; LORITO, M. 2006. The molecular biology of the interactions between Trichoderma spp, pathogenic fungi, and plants. Phytopathology. 96: 181 - 185.

Published

2016-03-10

How to Cite

Checa C., O. E., Descance V., J. A., Toro C., M. X., Álvarez, S. L., & Salazar G., C. (2016). Molecular characterizacion of Trichoderma spp. in pea Pisum sativum L. Revista De Ciencias Agrícolas, 32(2), 3–12. https://doi.org/10.22267/rcia.153202.8