Genetic diversity and geographical genetic diversity in Colombian accessions of Lippia alba (Mill.) N.E. Brown
DOI:
https://doi.org/10.22267/rcia.193602.117Keywords:
anthropochory, GD-GGD, mantel analysis, verbena complex, RAM, A-2X DNA protocol extractionAbstract
Bushy matgrass (Lippia alba, Verbenaceae) is a promising plant genetic resource, by their active compounds. The present document studied the molecular genetic diversity and spatial genetic structure of two contrasting populations of L. alba in Colombia. Eight RAM, evaluated total DNA of 59 accessions of non-cultivated plants collected in two Colombia regions, Chicamocha and Sumapaz. The expected average heterozygosity (or average heterozygosity genetic diversity of Nei) for the sample had low value (0.0≤He=0.2467≤0.5). The values of molecular diversity (MD) indicated values in the range of 0.1219 to 0.3425 for seven RAM. The frequency of variants is based on an effective number of alleles [Ae] and expected heterozygosity [He], genetic diversity by locus (hj=1-p2-q2) had maximum values (near 0.5) in the primers ACA, AG, CGA, and CEC. RAM suitably analyzed Lippia alba as an endemic genetic resource. A DNA bank composed of 59 Colombian accessions from Lippia alba was set up. The analysis of the spatial global structure shows that the subpopulation Sumapaz is structured, whilst the subpopulation Chicamocha, is in the structuring process. The results suggest in all cases the need for implementing: a) exchange of gene-seed, (b) gene banks with maximum genetic variability and c) induce genetic diversity.
Downloads
Metrics
References
Arcos, A. L.; Mondragón, A. J.; Muñoz, J. E.; Botero, S. (2004). Colecta y caracterización molecular con marcadores tipo RAM (Microsatélites aleatorios) de heliconias y especies relacionadas. Colombia: Congreso Colombiano de Botánica: Botánica, Diversidad y Cultura.
Bonilla, M. L.; Piedrahita, K. E.; Terranova, A. M. P.; Amariles, H. D. V.; Flórez, J. E. M. (2008). Caracterización molecular de 43 accesiones de uchuva de seis departamentos de Colombia. Acta Agronómica. 57(2): 109-115.
Budeguer, C. J.; Nasif, A.; Martínez Pulido, L.; Pastoriza, A.; Andrada Mansilla, B. (2013). Cytogenetics of Lippia alba (Mill.) Brown from Lules, Tucumán. Revista Agronómica del Noroeste Argentino. 33(1): 11-14.
Burokiene, D.; Prospero, S.; Jung, E.; Marciulyniene, D.; Moosbrugger, K.; Norkute, G.; Rigling, D.; Lygis, V.; Schoebel, C. N. (2015). Genetic population structure of the invasive ash dieback pathogen Hymenoscyphus fraxineus in its expanding range. Biological invasions. 17(9): 2743-2756.
Caetano, C. M.; Serna, C.; Yamil, D.; Muñoz Galindez, E. (2011). 10 años de la maestría en ciencias biológicas, línea de investigación en recursos Fitogenéticos Neotropicales: formando capacidades para contribuir a la conservación del patrimonio genético y la valoración del conocimiento tradicional. Recovered from https://repositorio.unal.edu.co/handle/unal/8361
Cardona Montoya, J. O. Estructura genética y fitogeografía de poblaciones colombianas de Lippia alba (Mill.) NE Brown (Verbenaceae). Recovered from http://www.bdigital.unal.edu.co/12886/
Dar, R. A.; Saba, I.; Shahnawaz, M.; Qazi, P. H.; Khan, I. A. (2017). Antimicrobial potential of fungal endophytes from selected high value medicinal plants of the Kashmir valley–India. The Journal of Psychopharmacology. 6(5): 307-310.
de Souza, E. M.; de Souza, R. C.; Melo, J. F.; da Costa, M. M.; de Souza, A. M.; Copatti, C. E. (2019). Evaluation of the effects of Ocimum basilicum essential oil in Nile tilapia diet: growth, biochemical, intestinal enzymes, hematology, lysozyme and antimicrobial challenges. Aquaculture. 504: 7-12.
do Amaral, U.; Pereira, M. B.; Junior, P.; de Souza, M.; Bajay, M. (2017). Polymorphism in Lippia alba clones from the metropolitan region of Rio de Janeiro. Journal of Advance in Agriculture. 7(2): 1044- 1049.
dos Santos, C. P.; de Oliveira, T. C.; Pinto, J.; Fontes, S.; Cruz, E.; Arrigoni-Blank, M.; Andrade, T.; de Matos, L. Alves, P.; Innecco, R.; Blank, A. F. (2015). Chemical diversity and influence of plant age on the essential oil from Lippia sidoides Cham. germplasm. Industrial Crops and Products. 76: 416-421.
Gharbi, Y.; Triki, M. A.; Jolodar, A.; Trabelsi, R.; Gdoura, R.; Daayf, F. (2014). Genetic diversity of Verticillium dahliae from olive trees in Tunisia based on RAMS and IGS-RFLP analyses. Canadian journal of plant pathology. 36(4): 491-500.
Gomide, M. D.; Lemos, F. D. O.; Lopes, M. T.; Tânia, M. D.; Viccini, L.; Coelho, C. M. (2013). The effect of the essential oils from five different Lippia species on the viability of tumor cell lines. Revista Brasileira de Farmacognosia. 23(6): 895-902.
Grover, A.; Sharma, P. C. (2016). Development and use of molecular markers: past and present. Critical reviews in biotechnology. 36(2): 290-302.
Herrera-Moreno, A. M.; Carranza, C. E.; Chacón-Sánchez, M. I. (2013). Establishment of propagation methods for growing promising aromatic plant species of the Lippia (Verbenaceae) and Tagetes (Asteraceae) genera in Colombia. Agronomía Colombiana. 31(1): 27-37.
Kosman, E.; Leonard, K. J. (2005). Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid, diploid, and polyploidy species. Molecular Ecology. 14(2): 415-424.
Manica-Cattani, M. F.; Zacaria, J.; Pauletti, G.; Atti-Serafini, L.; Echeverrigaray, S. (2009). Genetic variation among South Brazilian accessions of Lippia alba Mill. (Verbenaceae) detected by ISSR and RAPD markers. Braz. J. Biol. 69(3): 375-380.
Martínez-Natarén, D. A.; Parra-Tabla, V.; Ferrer-Ortega, M. M.; Calvo-Irabién, L. M. (2014). Genetic diversity and genetic structure in wild populations of Mexican oregano (Lippia graveolens HBK) and its relationship with the chemical composition of the essential oil. Plant systematics and evolution. 300(3): 535-547.
Martinez, F. (2008). Genetic Diversity Within and Among Wild and Garden Aromatic Species of the Genera Lippia, Aloysia and Phyla in Several Locations in Northeastern Colombia. Recovered from http://noesis.uis.edu.co/bitstream/123456789/30345/1/140155.pdf
Muñoz, J. E.; Coronado, A. C.; Coronado, Y. M. (2008). Microsatélites amplificados al azar (RAM) en estudios de diversidad genética vegetal. Acta agronómica. 57(4): 219-226.
Nath, V. S.; Basheer, S.; Jeeva, M. L.; Veena, S. S. (2016). Genetic and Phenotypic characterization of Phytophthora colocasiae in Taro Growing Areas of India. J. Plant Pathol Microbiol. 7(383): 2-7.
Ng, W.; Tan, S. G. (2015). Inter-simple sequence repeat (ISSR) markers: are we doing it right. ASM Sci J. 9(1): 30-39.
Peakall, R. O.; Smouse, P. E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Resources. 6(1): 288-295.
Pereira, I.; Aleksandrazielińska, F. J. V.; Santos, A.; Nowak, I.; Silva, A.; Souto, E. B. (2018). Monoterpenes-Based Pharmaceuticals: A Review of Applications in Human Health and Drug Delivery Systems. In: Goyal, M.; Chauhan, D. Plant-and Marine-Based Phytochemicals for Human Health: Attributes, Potential, and Use. pp. 85-130. Canada: CRC Press. 368p.
Pierre, P. M.; Sousa, S. M.; Davide, L. C.; Machado, M. A.; Viccini, L. F. (2011). Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae). Anais da Academia Brasileira de Ciências. 83(3): 993-1006.
Rasouli, M.; Martínez, G. P.; Karimi, R. (2015). Application of Random Amplified Microsatellite Polymorphism (RAMP) in Prunus characterization and mapping. Journal of Nuts. 6(01): 1-5.
Reis, A. C.; Sousa, S. M.; Vale, A. A.; Pierre, P. M.; Franco, A. L.; Campos, J. M.; Vieira, R. F.; Viccini, L. F. (2014). Lippia alba (Verbenaceae): A new tropical autopolyploid complex? American Journal of Botany. 101(6): 1002-1012. doi:10.3732/ajb.1400149
Rocha, D. S.; Santos, C. P. D.; Bajay, M. M.; Campos, J. B. D.; Blank, A. F.; Pinheiro, J. B.; Zucchi, M. I. (2015). Development of a novel set of microsatellite markers for Lippia alba (Verbenaceae). Recovered from https://www.repositorio.ufs.br/handle/riuf s/7289
Rodrigues, R. A. F.; Figueira, G. M.; Sartoratto, A.; Yamane, L. T.; de Freitas-Blanco, V. S. (2018). Chemical Diversity and Ethno pharmacological Survey of South American Medicinal and Aromatic Plant Species. In: Medicinal and Aromatic Plants of South America. pp. 17-44. Dordrecht: Springer.
Sala, C.; Ramos, M. L.; Bulos, M.; Altieri, E. (2017). U.S. Patent No. 9,574,237. Washington, DC: U.S. Patent and Trademark Office.
Shamim, M.; Kumar, P.; Kumar, R. R.; Kumar, M.; Kumar, R. R.; Singh, K. N. (2017). Assessing Fungal Biodiversity Using Molecular Markers. In: Molecular Markers in Mycology. pp. 305-333. Cham: Springer,
Sanabria, H. L. (2006). Caracterización molecular con marcadores RAM de árboles nativos de Psidium guajava (guayaba) en el Valle del Cauca. Acta Agronómica. 55(1): 23-30.
Santos, C. P.; Rocha, D. S.; Bajay, M. M.; Santos, F. R. C.; Campos, J. B.; Pinheiro, J. B.; Zucchi, M.; Silva- Mann, M.; Arrigoni-Blank, M.; Blank, A. F. (2014). Cross-species transferability of microsatellite markers in the genus Lippia. Genetics and Molecular Research. 13(4): 9846-9850.
Suárez, A R.; Martínez, F. O.; Núñez, G. A.; Castillo-Villamizar, G. A.; Chacón M I. (2007). Molecular characterization of aromatic species of the genus Lippia from the Colombian neotropics, Recovered from https://www.actahort.org/books/756/756_14.htm
Suárez, A R.; Castillo, G.A.; Chacón, M. I. (2008). Genetic diversity and spatial genetic structure within a population of an aromatic shrub, Lippia origanoides (Verbenaceae), in the Chicamocha Canyon, northeastern Colombia, Genetics Research. 90(6): 455-465.
Venâncio, D.; Viccini, L.; Luizi-Ponzo, A.; Prezoto, F. (2016). Flower-Visiting Insects and Phenology of Lippia alba (Lamiales: Verbenaceae): Floral Color Changes and Environmental Conditions as Cues for Pollinators. Environmental entomology. 45(3): 685-693.
Vega-Vela, N. E.; Chacón, M. I. (2011). Isolation of high-quality DNA in 16 aromatic and medicinal Colombian species using silica-based extraction columns. Agronomía Colombiana. 29(3): 349-357.
Viccini, L.F., Silveira, R.S.; do Valea, A.A.; de Campos, J.M.; Reis, A.C.; de Oliveira Santos, M.; Campos, V.R.; Carpanez, A.G.; Grazul, R.M. (2014). Citral and linalool content has been correlated to DNA content in Lippia alba (Mill.) NE Brown (Verbenaceae). Industrial Crops and Products. 59: 14-19.
Yuan, Y. W.; Liu, C.; Marx, H. E.; Olmstead, R. G. (2010). An empirical demonstration of using pentatricopeptide repeat (PPR) genes as plant phylogenetic tools: Phylogeny of Verbenaceae and the Verbena complex. Molecular Phylogenetics and Evolution. 54(1): 23-35.
Zarroug, M. B.; Baraket, G.; Zourgui, L.; Souid, S.; Hannachi, A. S. (2015). Genetic diversity and phylogenetic relationship among Tunisian cactus species (Opuntia) as revealed by random amplified microsatellite polymorphism markers. Genetics and Molecular Research. 14(1): 1423-1433.
