Research Article
Vol. 40 No. 2 (2023): Revista de Ciencias Agrícolas - Mayo - August 2023
Tetrazolium test to estimate the physiological quality of tamarind (Tamarindus indica L.) seeds
Abstract
Tamarind (Tamarindus indica) is a fruit tree of African origin cultivated in more than 50 countries, including Colombia, mainly in the Colombian Caribbean departments, where its harvest generates significant income in rural communities. Its establishment presents a difficulty because the sexual seed presents physical dormancy and poor germination. The objective of this study was to evaluate Tamarind seed viability by using the tetrazolium test and to reduce the time needed for the determination of its physiological quality. Ripe and healthy fruits were collected from patio trees, from which their seeds were extracted, disinfected with 1% sodium hypochlorite, and washed with plenty of water before use. Subsequently, they were scarified with No. 100 sandpaper by the edges, except in the area of the micropyle. The completely random design was used in a 3x3 bifactorial arrangement, with four repetitions. Thefirst factor, tetrazolium concentration (%), was tested at levels of 0.50, 0.75and 1.00%, and the second factor, immersion time, at 2, 4, and 6 hours. The analysis of variance did not show significance for the tetrazolium concentration, while it did for the immersion time(p≤0.01), and the tetrazolium-time interaction(p≤0.05). Therefore, the concentration of 0.50% tetrazolium for six hours of immersion is a reliable alternative to determine the physiological quality of tamarind seeds compared to conventional germination testing due to its low cost and execution time.Likewise, the percentages of germinationand germination speed index were higher when the seed was scarified with sandpaper than the conventional method.
References
- Azad, S. (2018). Tamarindo-Tamarindus indica. In: Rodrigues,S.;Silva, E.;de Brito, E. Exotic Fruits Reference Guide.pp 403-412. Brasil: Academic Press.10.1016/B978-0-12-803138-4.00055-1
- Bahru, T.; Eshete, A.; Mulatu,Y.; Kebede, Y.;Tadesse, W.; Mohammed, O.; Dejene, T. (2014). Effect of provenances on seed germination, early survival and growth performance of Tamarindus indica l. in Ethiopia: a key multipurpose species. Advances in Materials Science and Engineering. An International Journal.1(1):1-8.
- Bello, A.G.; Gad, Z.Y.(2015). Germination and Early Growth Assessment of Tamarindus indicaL in Sokoto State, Nigeria. International Journal of Forestry Research. Article. 2025(63410): 5.http://dx.doi.org/10.1155/2015/634108
- Besaye, B.H.; Galgaye, G.G. (2022). Impact of common bean (Phaseolus vulgarisl.) genotypes on seed yield, and seed quality at different locations of Eastern Ethiopia. Food & Agriculture. 8(1): 2115674. 10.1080/23311932.2022.2115674
- Bhattacharya, E.; Suparna, M.B. (2021). Role oftartaric acid in the ecology of a zoochoric fruit species, Tamarindus indicaL. International Journal of Fruit Science. 21(1):819-825.10.1080/15538362.2021.1936347
- Brito, W.A.; Pereira, K.T.; Nogueira, N.A.; Torres, S.; Paiva, E. (2020). Evaluation of viability of Tabebuia aurea seeds through tetrazolium test. Revista Caatinga. 33(4): 993-999.10.1590/1983-21252020v33n414rc
- Carvalho, S.M.; Torres, S.B.; Sousa, E.C.; Sousa, D.M.; Pereira, K.T.; Paiva, E.P.; Matias, J.R.;Santos, B.R. (2018). Viability of Carica papayaL. Seeds by the Tetrazolium Test. The Journal of Agricultural Science.10.335-340.10.5539/jas.v10n2p335
- Cordeiro, L.N.; Bruno, R.D.L.A.; Lima, L.K.S.; De Andrade, A.P.; Da Silva Belarmino, K.; Alves, E.U. (2022). Suitability of tetrazolium test for Tamarindus indicaL. seeds. Semina: Ciências Agrárias.43(2): 509-522.10.5433/1679-0359.2022v43n2p509
- Costa, M.A.; Shimizu, E.S.; Leão, N.V.; Pinheiro, H.A. (2018). Seed quality evaluation by tetrazoliumstaining of Parkia multijugaBenth. Agricultural Sciences. 9:1577-1586.10.4236/as.2018.95040
- Fandohan, B.; Assogbadjo, A.E.;Kakai, R.G.; Kyndt, T.; Calwé, E.; Codjia, J.T.; Sinsin, B. (2019).Women’s Traditional Knowledge, Use Value,and the Contribution of Tamarind (Tamarindus indicaL.) to Rural Households’ Cash Income inBenin. Economic Botany. 64(3): 248-259. http://dx.doi.org/10.1007/s12231-010-9123-2
- Franca-Neto, J.; Krzyzanowski, F.C. (2019). Tetrazolium: an important test for physiologicalseed quality evaluation. Journalof Seed Science.41(3): 359-366. https://doi.org/10.1590/2317-1545v41n3223104
- Gomes, C.D.; De Sa, J.M.; Rodrigues, M.H.; Sousa,V.F.; Bomfim, M.P. (2019). Production of Tamarindus indicaL. seedlings submitted to substrates and pre-germination methods. Pesq. Agropec. Trop.49: e54029. https://doi.org/10.1590/1983-40632019v4954029
- Govindappa, M.; Birawat, R.; Raghavendra, V.; Munawer, U.; Ningaraju, S.;Al-Rashed, S.; Chowdappa, S.; Pugazhendh, A.;Akshatha, K.(2021).In vitro therapeutic evaluation of nanoliposome loaded with Xyloglucans polysaccharidesfrom Tamarindus flower extract. International Journal of Biological Macromolecules. 178:283-295. 10.1016/j.ijbiomac.2021.02.140
- Guevara-Ohara, J.E.; Cardozo-Conde, C.I.; Santos-Meléndez, L.G. (2020). Caracterización morfo-anatómica y protocolo para estimar la viabilidad de semillas de Psidium guajava. Acta Agronómica. 69(2):155-161. https://doi.org/10.15446/acag.v69n2.65257
- Liang, H.; Xiong, Y.; Guo, B.; Yan, H.; Jian, S.; Ren, H.; Zeng, S.; Wu, K.; Silva, J.; Xiong, Y.; Ma, G. (2020). Effective breaking of dormancy of Scaevola sericeaseeds with seawater, improved germination, and reliable viability testing with 2,3,5-triphenyl-tetrazolium chloride. South African Journal of Botany.132:73-78. 10.1016/j.sajb.2020.04.018
- López, C.R.; Sarmiento, C.; Espitia, L.; Barrero, A.M.; Consuegra, C.; Gallego, C.B. (2016). 100 plantas del Caribe colombiano. Usar para conservar: aprendiendo de los habitantes del bosque seco. Bogotá D.C. Colombia: Fondo Patrimonio Natural. 240 pp.
- Maguire, J. D. (1962). Speed of germination-aid in selection and evaluation for seedlingemergence and vigor. Crop Sci.2(2): 176-177.10.2135/cropsci1962.0011183X000200020033x
- Maia, J.L.; Wurlitzer, N.J.; Lima, J.R.; Borges, M.F.;Maia, M.O.; Damião, B.S.; Oliveira, L.S. (2021). Quality and storage stability of tamarind juice. Brazilian Journal of Food Technology. 24:e2020027.10.1590/1981-6723.02720
- Mancipe-Murillo, C.; Calderón-Hernández, M.; Pérez-Martínez, L. (2018). Evaluación deviabilidad de semillas de 17 especies tropicalesaltoandinas por la prueba de germinación y laprueba de tetrazolio. Caldasia.40(2):366-382.10.1544 6/caldasia.v40n2.68251
- Marcos-Filho, J. (2015). Fisiología de sementes deplantas cultivadas. 2 edition. Londrina: Editorial ABRATES. 660p.
- Okello, J.; John Bosco, L.; Eilu, G.; Nyeko, P.; Obua, J. (2018). Morphological variations in Tamarindus indicaLINN. Fruitsand Seed Traits in the Different Agroecological Zones of Uganda. International Journal of Ecology. ID8469156. 10.1155/2018/8469156
- Paiva, E.; Torres, S.; Almeida, J.P.; Sá, F.; Oliveira, R.R. (2017). Tetrazolium test for the viability of gherkin seeds. RevCiência Agronômica.48(1):118-124.http://dx.doi.org/10.5935/1806-6690.20170013
- Pereira, K.T.; Benedito, C.P.; Paiva, E.P.; Souza Neta, M.L.; Torres, S.B. (2020). Physiologicalquality evaluation of Piptadenia stipulacea(Benth.) Ducke seeds by tetrazolium test. RevCiência Agronômica.51(1):e20196712. 10.5935/1806-6690.20200019
- Salazar, S.A.; Botello, E.A.; Quintero, J.D. (2020). Optimización de la prueba de tetrazolio para evaluar la viabilidad en semillas de Solanum lycopersicumL. Revistade Ciencia y Tecnología Agropecuaria.21(3):e1344. https://doi.org/10.21930/rcta.vol21_num3_art:1344
- SAS-Institute Inc. (2013). SAS/STAT 9.4 User's Guide. Cary, NC: SAS Institute Inc.Silva, G.P.; Sales, J.; Zuchi, J.; Telles, K.J.; Vinhais, M.M.; Rodrigues, A.A. (2021). Physiological Quality of Stored Dipteryx alataVogel Seeds: Tetrazolium Test and Radiographic Imaging Standardization. Floresta e Ambiente. 28(3): e20200022. https://doi.org/10.1590/2179-8087-FLORAM-2020-0022
- Souza, A.D.; Nogueira, N.; Torres, S.; Benedito, C.P.; De Souza, M.; De Paiva, E. (2018). Tetrazoliumtest to estimate okra (Abelmoschusesculentus(L.) MOENCH) seed viability. Rev.Caatinga. 31(4): 817822. https://doi.org/10.1590/1983-21252018v31n403rc
- Yousif, M.A.; Wang, Y.R.; Dali, Ch. (2020). Seeddormancy overcoming and seed coat structure change in Leucaena leucocephalaand Acacianilótica. Forest Science and Technology.6(1):18-25. 10.1080/21580103.2019.1700832
- Zuffo, A.M.; Steiner, F.; Busch, F.; Zuffo-Júnior, J.M.; Mendes, A.; Oliveira, N.; Zambiazzi, E.(2018). Quality of soybean seeds in response to nitrogen fertilization and inoculation with Bradyrhizobium japonicum. Pesqui.Agropecu.Trop.Goiânia. 48(3): 261-270.10.1590/1983-40632018v4851638
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