Nematicide evaluation of the essential oil from Tagetes zypaquirensis in the control of the nematode Meloidogyne spp.
DOI:
https://doi.org/10.22267/rcia.163301.3Keywords:
Root-knot, essential oil, dihydrotagetone, E-tagetone, Solanum quitoense Lam.Abstract
Lulo (Solanum quitoense Lam.), an Andean fruit, is economically important in Colombia, however, cultivated areas as well as yields, have suffered a considerable reduction by the attack of pathogens such as the rootknot nematode Meloidogyne spp. with losses near to 50%. In nature, there are different plant with nematicides properties which could diminish the effects of this nematode, such as the Tagetes genre. The objective of this research was to evaluate the nematicidal activity of the essential oil from Tagetes szypaquirensis on Meloidogyne spp. Four concentrations of essential (100, 200, 400, 800mg/kg of soil) were evaluated under greenhouse conditions. These doses were applied to nematodes in the second instar approximately +400 J2/100g of soil. Moreover, three treatments were established: the first with no application of essential oil, the second without nematode, and the third with soil treated with carbofuran (33.2%) at dose of 2cc/L. For each treatment the variables evaluated were: level of severity, plant height, fresh and dry weight, fresh root weight, number of nematode eggs/100g roots and number of J2/100g of soil. The main components of the essential oil from T. zypaquirensis were dihydrotagetone and E-tagetone with a composition of 42.2% and 22.9% respectively. The results indicated that a concentration 800mg/kg of essential oil had the same nematicidal action than soil treated with carbofuran, same population constrains and phitometric variables. It was concluded that T. zypaquirensis essential oil can be an alternative for managing root knot disease.
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ADEGBITE, A.; ADESIYAN, O. 2006. Root extracts of plants to control root knot nematode on edible Soybean (Peer reviewed papers). Journal of Vegetable Science. 12(2):5 - 12.
ADEKUNLE, O.; ACHARYA, R.; SINGH, B. 2007. Toxicity of pure compounds isolated from Tagetes minuta oil to Meloidogyne incognita. Australasian Plant Disease. 2:101 - 104.
ALVAREZ, D.; CASANOVA, L.; CÓRDOBA, K.; OSORIO, O. 2016. Evaluación de postcosecha y calidad fisicoquímica de genotipos de lulo (Solanum quitoense Lam.) tolerantes a Meloidogyne spp. Vitae. 23(1):785.
BARBOSA, F.; BARBOSA, L.; MELO, E.; BOTELHO, F.; SANTOS, R. 2006. Influência da temperatura do ar de secagem sobre o teor e a composição química do óleo essencial de Lippia alba (Mill). Química Nova. 15 (1):1 - 5.
CASTRO, L.; FLORES, L.; URIBE, L. 2011. Efecto del vermicompost y quitina sobre el control de Meloidogyne sp. en tomate a nivel de invernadero. Agronomía Costarricense. 35(2):21 - 32.
CAVENESS, F.; JENSEN, J. 1955. Modification of the centrifugal flotation technique for the isolation and concentration of nematodes and their eggs from soil and plant tissue. Journal of the Helminthological Society of Washington. 22:87 - 89.
CHAVES, J.; GAVIRIA, F.; TORRES, F.; OBANDO, L. 2002. Análisis de algunos aspectos agroeconómicos del cultivo del lulo (Solanum quitoense L.). Rev. Cienc. Agri. 14(2):178 - 188.
CHITWOOD, J. 2002. Phytochemical based strategies for nematode control. Annual Review of Phytopathology. 40:221 - 249.
DÍAZ, F.; SERRATO, M.; ARCE, M.; LEÓN, J. 2012. Composición del aceite esencial de Tagetes lacera, planta endémica de Baja California del Sur, México. Revista Mexicana de Biodiversidad. 83:543 - 547.
DI RIENZO, A.; CASANOVES, F.; BALZARINI, M.; GONZÁLEZ, L.; TABLADA, M.; ROBLEDO, W. 2013. InfoStat versión 2013. En: http://www.infostat.com.ar; consulta: agosto, 2015.
EL-SHERIF, G.; EFAEI, R.; NAGAR, E.; SALEM, M. 2007. The role of eggs inoculum level of Meloidogyne sp., on their reproduction and host reaction. African Journal of Agricultural Research. 2(4):159 - 163.
ELYOUSR, A.; KAMAL, A.; ZAKAULLAH, K.; MAGD, E.; MONTASER, F. 2010. Evaluation of plant extracts and pseudomonas spp. For control of root knot nematode, Meloidogyne sp., on tomato. Nematropica. 40(2):12.
ESCOBAR, P.; HERRERA, L.; LEAL1, S.; DURÁN, C.; STASHENKO, E. 2009. Composición química y actividad antitripanosomal de aceites esenciales obtenidos de Tagetes (Fam. Asteraceae), recolectados en Colombia. Revista Salud Universidad Industrial de Santander. 41:280 - 286.
GELPUD, C.; MORA, E.; SALAZAR, C.; BETANCOURTH, C. 2011. Susceptibilidad de genotipos de Solanum spp., al nematodo causante del nudo radical Meloidogyne spp. (chitwood). Acta Agronómica. 60(1):50 - 67.
KIMPINSKI, J.; ARSENAULT, C.; GALLANT, A.; SANDERSON J. 2000. The effect of marigolds (Tagetes sp.) and other cover crops on Pratylenchus penetrans and on following potato crops. Journal of Nematology. 32:531 - 536.
KUMAR, S.; KHANNA, A. 2006. Effect of neem-based products on the root-knot nematode, Meloidogyne sp., and growth of tomato. Nematologia Mediterranea. 34:141 - 146.
LEHMAN, S. 1979. Factors influencing nematode control with Marigolds. En: http://www.freshfromflorida.com/content/download/10848/141379/nem050.pdf; consulta: noviembre, 2013.
MURCIA, M.; STASHENKO, E. 2008. Determinación de plaguicidas organofosforados en vegetales producidos en Colombia. Agro Sur. 36(2):11.
MÚ NERA, E. 2002. Nematodos asociados con el cultivo del lulo. pp. 135-141. En: Memorias IV Seminario de Frutales de Clima Frío Moderado. Medellín. Colombia.
MADR. MINISTERIO DE AGRICULTURA Y DESARROLLO RURAL. 2012. Anuario estadístico de frutas y hortalizas 2012 y sus calendarios de siembras y cosechas. En: http://207.239.251.112/www/htm3b/public/Anuario/Anuario_Estadistico2012.pdf; consulta: febrero, 2014.
MADR. MINISTERIO DE AGRICULTURA Y DESARROLLO RURAL. 2013. Anuario estadístico de frutas y hortalizas 2013. En: http://207.239.251.112/www/htm3b/public/Anuario/ANUARIO_2013.pdf; consulta: febrero, 2015.
OKA, Y.; NACAR, S.; PUTIEVSKY, E.; RAVID, U.; YANIV, Z.; SPIEGEL, Y. 2000. Nematicidal activity of essential oils and their components against the root knot nematode. Phytopathology. 90:710 - 715.
PARADA, R.; GUZMÁN. F. 1997. Evaluación de extractos botánicos contra el nematodo Meloidogyne sp., en frijol (Phaseolus vulgaris). Agronomía Mesoamericana. 8(1):108 - 114.
PÉREZ, M.; CORTÉS, A.; VILLALOBOS, J.; CASTILLO, P. 2003. Nematicidal activity of essential oils and organic amendments from Asteraceae against root knot nematodes. Plant Pathology. 52:395 - 401.
PLOEG, T. 2000. Effects of amending soil with Tagetes patula cv. single gold on Meloidogyne sp., infestationof tomato. Nematology. 2:289 - 493.
QUINTERO, A.; GONZÁLEZ, N. 2004. Aceite esencial de las hojas Hyptis umbrosa Salzm, extraído por diferentes técnicas. Acta Científica Venezolana. 55:181 - 187.
RODRÍGUEZ, J.; FERNÁNDEZ, J.; REBOLLEDO, L.; URIZA, D. 2001. Uso de extractos vegetales para el combate del nematodo agallador (Meloidogyne Sp.) de la piña (Ananas Comosus (L.) Merr.) en el campo experimental cotaxtla del inifap. p.59. En: Decima Cuarta Reunión Científica-Tecnológica Forestal y Agropecuaria. Veracruz. Mexico.
RIGA, J. 2005. Root extracts of plants to control root knot nematode on edible Soybean (Peer reviewed papers). Journal of Vegetable Science. 12(2):5 - 12.
SERRATO, M.; BARAJAS, J.; DÍAZ, F. 2007. Aceites esenciales del recurso genético Tagetes para el control de insectos, nematos, ácaros y hongos. Agricultura sostenible. 3:181 - 192.
STEFANAZZI, N.; GUTIERREZ, M.; STADLER, T.; BONONI, N.; FERRARI, A. 2006. Actividad biológica del aceite esencial de Tagetes terniflora Kunth (Asteraceae) en Triboleum castaneum Herbst (insecta, coleóptera, tenebrionidae). Boletín de sanidad vegetal de plagas. 32:439 - 447.
TAMAYO, P.; NAVARRO, R.; FORERO, R. 2003. Enfermedades del Lulo en Colombia: Guía de Diagnóstico y Control. Boletín Técnico No 9. Corpoica. 48 p.
TALAMINI, V.; STADNIK, J. 2004. Extractos vegetales e de algas no controle de doenças de plantas. Manejo ecológico de doenças de plantas. 1:45 - 62.
TAYLOR, A.; SASSER, J. 1983. Biología, identificación y control de nematodos del nudo de la raíz (especies de Meloidogyne). Primera edición. Artes gráficas de la Universidad del estado de Carolina del Norte. 111 p.
TSAY, T; WU, S.; LIN, Y. 2004. Evaluation of Asteraceae plants for control of Meloidogyne sp., Journal of Nematology. 36(1):36 - 41.
