Molecular characterization of cubios (Tropaeolum tuberosum Ruíz and Pavón) in the department of Boyacá

Keywords: Tropaeloaceae, microsatellite RAMs, genetic diversity, conservation, germplasm.

Abstract

The cubio (Tropaeolum tuberosum Ruíz and Pavón) is a basic tuber in the diet of Andean communities. It is a rustic crop, with medicinal uses. Farmers in the highlands of Boyacá department conserve tuber species such as cubios, rubas and ibias, as part of their cultural heritage; however, in recent years this germplasm has been threatened by genetic erosion due anthropogenic  and environmental factors.  There are not studies of molecular characterization  of this germplasm in Boyacá,  thus this research aims to identify the genetic diversity of eleven genotypes of cubios from Ventaquemada and San Pedro de Iguaque, using seven Random Amplified Microsatellite markers (RAMs). The similarity analysis differentiated the materials into two main groups, according to characteristics related to the tuber’s color and shape. The estimated heterozygosity values ranged between 0.25 and 0.42 for CGA and AG primers, respectively, which is low when compared to the results obtained in other tuberous species. The heterozygosity average in the population was 0.35, and together with the analysis of molecular variance and Fst show the existence of genetic variability in the cultivars evaluated, which should be considered in conservation programs and plant breeding of Andean tubers.

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References

AGUIRRE, S.; PIRANEQUE, N.; PÉREZ, I. 2012. Sistema de producción de tubérculos andinos en Boyacá, Colombia. Cuadernos de Desarrollo Rural. 9(69): 257 - 273.

BORNET, B.; GORAGUER, F.; JOLY, G.; BRANCHARD, M. 2002 Genetic diversity in European and Argentinian cultivated pótateles (Solanum tuberosum ssp. Tuberosum) detected by inter-simple sequence repeats (ISSRs). Genome. 45(3):481 - 484. doi: 10.2225/vol16-issue6-fulltext-15.

CADIMA, F. 2006. Tubérculos. In. R. Moraes, B. Ollgaard, L.P. Kvist, F. Borchsenius and H. Balslev H (ed.) Botánica Económica de los Andes Centrales. Universidad Mayor de San Andrés, La Paz, Bolivia. 347 - 369 p.

CLAVIJO, N.; COMBARIZA, J.; BARÓN, M. 2011. Recognizing rural territorial heritage: characterization of Andean tuber production systems in Boyacá. Agronomía Colombiana. 29(2):315 - 322.

CHIRINOS, R.; CAMPOS, D.; COSTA, N.; ARBIZU, C.; PEDRESCHI, R.; LARONDELLE Y. 2008a. Phenolic profiles of Andean mashua (Tropaeolum tuberosum Ruíz and Pavón) tubers: Identification by HPLC-DAD and evaluation of their antioxidant activity. Food Chemistry. 106(3):1285 - 1298.

CHIRINOS, R.; CAMPOS, D.; WARNIER, M.; PEDRESCHI, R.; REES, J.; LARONDELLE, Y. 2008b. Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays. Food Chemistry. 111(1):98 - 105.

CHIRINOS, R.; ROGEZ, H.; CAMPOS, D.; PEDRESCHI, R.; LARONDELLE, Y. 2007. Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruíz y Pavón) tubers. Separation and Purification Technology. 55(2):217 - 225.

DELLAPORTA, S.; WOOD, J.; HICKS, J. 1983. A plant DNA minipreparation: Versión II. Plant Molecular Biology Reporter. 1 (4):19 - 21.

ELIAS, M.; PANAUD, O.; ROBERT, T. 2001a. Traditional management of cassava morphological and genetic diversity by the Makushi Amerindians (Guyana, South America): Perspectives for on-farm conservation of crop genetic resources. Euphytica. 120(1):143 - 157. doi: 10.1023/A:1017501017031.

ELIAS, M.; PENET, L.; VINDRY, P.; MCKEY, D.; PANAUD, O.; ROBERT, T. 2001b. Unmanaged sexual reproduction and the dynamics of genetic diversity of a vegetatively propagated crop plant, cassava (Manihot esculenta Crantz), in a traditional farming system. Molecular Ecology. 10(8):1895 - 1907. doi: 10.1046/j.0962-1083.2001.01331.x.

FLORES, H.; WALKER, T.; GUIMARAES, R.; BAIS, H.; VIVANCO, J. 2003. Andean root and tuber crops: Underground rainbows. Horticulture Science. 38 (2):161 - 167.

GRAU, A.; ORTEGA, R.; NIETO, C.; Hermann, M. 2003. Mashua (Tropaeolum tuberosum Ruiz & Pav.). Promoting the conservation and use of underutilized and neglected crops. International Potato Center, Lima. International Plant Genetic Resources Institute (IPGRI), Rome, Italy. 124 p.

IRIONDO, J.; MAXTED, N.; DULLOO, E. 2008. Conservating plant genetic diversity in protected areas: Population management of crop wild relatives. CABI Publishing, Wallingford, 144p.

JARAMILLO, L. 1999. Módulo I: Ciencia, tecnología, sociedad y desarrollo. En: Instituto Colombiano para el Fomento de la Educación Superior, ICFES (ed.). Santa Fe de Bogotá: ARFO. 145 p.

JOSHI, S.P.; GUPTA, V.S.; AGGARWAL, R.K.; RANJEKAR, P.K.; BRAR, D.S. 2000 Genetic diversity and phylogenetic relationship as revealed by intersimple sequence repeat ?ISSR? polymorphism in the genus Oryza. Theoretical and Applied Genetics. 100(8):1311 -1320. doi: 10.1007/s001220051440.

MALICE, M.; BIZOUX, J.; BLAS, R.; BAUDOIN, J. 2010. Genetic diversity of Andean tuber crop species in the in situ microcenter of Huanuco, Perú. Crop Science. 50(5):1915 - 1923. doi:10.2135/cropsci2009.09.0476.

MANRIQUE, I.; ARBIZU, C.; VIVANCI, F.; GONZÁLEZ, R.; RAMÍREZ, C.; CHÁVEZ, O.; TAY, D.; ELÍS, D. 2013. Tropaeolum tuberosum Ruíz and Pav. Colección de germoplasma de mashua conservada en el Centro Internacional de la Papa (CIP). Primera Edición. Centro Internacional de la Papa, La Molina, Perú. 34 - 77 p.

MONTEROS, A.; MAZO, N.; CASTILLO, R. 1997. Estudio de la variación morfológica e isoenzimática de 78 entradas de mashua (Tropaeolum tuberosum) en Santa Catalina, INIAP. 34 p.

MORILLO, A.; MORILLO, Y.; LEGUIZAMO, M. 2016. Diversidad genética de ibias Oxalis tuberosa y de cubios Tropaelum tuberosum Ruíz y Pavón en Boyacá. Temas agrios. 21 (1):44 - 53.

MORILLO, A.; MORILLO, Y.; PINZÓN, H. 2015. Molecular characterization of the plum collection (Prunus domestica L. Borkh) of the Pedagogical and Technological University of Colombia. African Journal of Biotechnology. 14(3):257 - 263. doi: https://dx.doi.org/10.5897/AJB2014.14003.

MOSCOE, L.; EMSHWILLER, E. 2015. Diversity of Oxalis tuberosa Molina: a comparison between AFLP and microsatellite markers. Genetic Resources and Corp. Evolution. 62(3): 335-347. doi: 10.1007/s10722-014-0154-x.

NEI, M.; LI, W. 1979. Mathematical model for studying genetic variation in terms of restriction endonuclease. Proceedings of the National Academy Sciences. 79(10):5267 - 5273.

NAVARRO, C.; BOLAÑOS, L.; LAGOS, T. 2010. Caracterización morfoagronómica y molecular de 19 genotipos de papa guata y chaucha (Solanum tuberosum L. y Solanum phureja Juz et Buk) cultivados en el departamento de Nario. Revista de Agronomía. 27(1):27 - 39.

OKPUL, T.; MACE, E.S.; GODWIN, I.D.; SINGH, D.; WAGIH, ME. 2005. Evaluation of variability among breeding lines and cultivars of taro (Colocasia esculenta) in Papua New Guinea using ISSR fingerprinting and agro-morphological characterization. Plant Genetics Resources Newsletter. 143(1):8 - 16.

ORTEGA, O.; DURAN, E.; ARBIZU, R.; ROCA, W.; POTTER, D.; QUIROS, C. 2007. Pattern of genetic diversity of cultivated and non-cultivated mashua, Tropaeolum tuberosum, in the Cusco region of Perú. Genetic Resource Crop Evolution. 54 (4):807 - 821. doi: 10.1007/s10722-006-9160-y.

PISSARD, A.; GHISLAIN, M.; BERTIN, P. 2006. Genetic diversity of the Andean tuber-bearing species, oca ?Oxalis tuberosa Mol.?, investigated by inter-simple sequence repeats. Genome. 49(1):8 - 16. Doi: 10.1007/s10709-007-9150-9.

PISSARD, A.; ARBIZU, C.; GHISLAIN, M.; BERTIN, P. 2008a. Influence of the geographical provenance on the genetic structure and diversity of the vegetatively propagated Andean tuber crop, mashua (Tropaeolum tuberosum), highlighted by ISSR markers and multivariate analysis methods. International Journal of Plant Sciences. 169(9):1248 - 1260. doi: 10.1086/591979.

PISSARD, A.; ARBIZU, C.; GHISLAIN, M.; FAUX, A.; PAULET, S.; BERTIN, D. 2008b. Congruence between morphological and molecular markers inferred from the analysis of the intramorphotype genetic diversity and the spatial structure of Oxalis tuberosa. Molecular Genetics. 132(1):71 - 85.

PISSARD, A.; ROJAS, J.; FAUX, A.; PAULET, S.; BERTIN, P. 2008c. Evidence of intra-varietal genetic variability in the vegetatively propagated crop oca (Oxalis tuberosa Mol.) in the Andean traditional farming system. Plant Systematics and Evolution. 270(1-2):59 - 74. doi: 10.1007/s00606-007-0605-3.

RUAS, P.; RUAS, C.; RAMPIM, L.; CARVALHO, V.; RUAS, E.; SERA, T. 2003. Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (inter-simple sequence repeats) markers. Genetics and Molecular Biology. 26(3):319 - 327. doi: 10.1590/S1415-47572003000300017.

SNEATH, P.; SOKAL, R.1973. Numerical Taxonomy: The Principles and Practice of Numerical Classification. W.H. Freeman and Co. San Francisco. 573 p.

SUKHOTU, T.; KAMIJIMA, O.; HOSAKA, K. 2005. Genetic diversity of the Andean tetraploid cultivated potato (Solanum tuberosum L. ssp. Andigena Hawkes) evaluated by chloroplast and nuclear DNA markers. Genome. 48(1):55 - 64. Doi: 10.1139/g04-086.

VERAMENDI, S.; CADIMA, X.; GABRIEL, J. 2013. Integración molecular y morfológica para la formación de la Colección Núcleo de papa de Bolivia. Revista Latinoamericana de la Papa. 17(2): 23 - 39.

WRIGHT, S. 1978. Evolution and the genetics of populations, variability within and among natural populations, Vol 4. University of Chicago Press. Chicago. 144 p.

ZHEDI, S.; SAKKA, H.; OULD, A.; RHOUMA, A.; MARRAKCHI, M.; TRIFI, M. 2005. Molecular polymorphism and genetic relationships in a Tunisian date-palm (Phoenix dactylifera L.) collection using ISSR amplification fingerprinting. Plant Genetics Resources Newsletter. 144(1):39 - 44.

Published
2016-12-14
How to Cite
Morillo C., A. C., Morillo C., Y., & Tovar L., Y. (2016). Molecular characterization of cubios (Tropaeolum tuberosum Ruíz and Pavón) in the department of Boyacá. Revista De Ciencias Agrícolas, 33(2), 32-42. https://doi.org/10.22267/rcia.163302.50
Section
Research and scientific and technological innovation article