Comparison of sampling methods to evaluate sugarcane root system

  • Adriana Marcela Silva-Olaya
  • Carlos Eduardo Pellegrino Cerri
  • Carlos Clemente Cerri
Keywords: Probe, root, root biomass, Saccharum officinarum.


The knowledge about the architecture of sugarcane root system is limited due to difficulties associated to methodological aspects. The correct way to assess the root system is still not clearly established. This study aimed to compare two direct methods of root biomass evaluation: soil monoliths and root sampling from probes. Three experimental plots (7.2 m2) were randomly established in an area under sugarcane planting on Typic Quartzipsamments located in São Pedro, Sao Paulo state, Brazil. One plant in each experimental plot was selected for root system assessment. The sampling with probes was performed in nine points inside half of the Voronoi polygon and the root density was estimated in nine combinations of sampling points. The root samples collected by the two methods were separate from soil particles by washing in a set of sieves. Afterwards, the roots were oven-dried and weighed. Significant differences (p<0.05) in the density root were found among the soil layers in each treatment. A trend of decreasing root density with soil depth was found in both methods. The probes method tended to underestimate the amount of root found in the soil top layer. Only two sampling combinations with probe indicated to be equivalent to the monolith method.


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ABAYOMI, Y.A. 1989. Effect of soil type and crop cycle on root development and distribution pattern of a commercial sugarcane cultivar under normal irrigation and field conditions at Bacita Estate, Nigeria. Turrialba. Scientific Research. 39:78 - 84.

ALLMARAS, R. R.; KRAFT, J. M.; MILLER, D. E. 1988. Effects of soil compaction and incorporated crop residue on root health. Annual Review Phytopathology. 26:219 - 243.

ALVAREZ, I. A.; CASTRO, P. D. C.; NOGUEIRA, M. C. S. 2000. Crescimento de raízes de cana crua e queimada em dois ciclos. Scientia Agricola. 57:653 - 659. Doi: 10.1590/S0103-90162000000400009.

BALL-COELHO, B.; SAMPAIO, E. V. S. B.; TIESSEN, H.; STEWART, J. W. B. 1992. Root dynamics in plant and ratoon crops of sugar-cane. Plant and Soil. 142:297 - 305. doi: 10.1007/BF00010975.

BOLINDER, M. A.; ANGERS, D. A.; DUBUC, J. P. 1997. Estimating shoot to root ratios and annual carbon inputs in soils for cereal crops. Agriculture Ecosystems & Environment. 63:61 - 66. Doi: 10.1016/S0167-8809(96)01121-8.

CAMPBELL, C. A.; ZENTNER, R. P.; LIANG, B. C.; ROLOFF, G.; GREGORICH, E. C.; BLOMERT, B. 2000. Organic C accumulation in soil over 30 years in semiarid southwestern Saskatchewan - Effect of crop rotations and fertilizers. Canadian Journal of Soil Science. 80:179 - 192. Doi: 10.4141/S99-028.

CASAGRANDE, A. A. 1991. Topicos de morfologia e fisiologia da cana-de-açucar. Funep. Jaboticabal. 157p.

CONAB CNDA. 2015. Acompanhamento da safra brasileira Cana-de-açúcar. Safra 2014/2015. Ed. Agronegócio Ddpaei-Sdid, CONAB. Brasilia. 1 - 29p.

EVANS, H. 1938. Studies on the Absorbing Surface of Sugar-cane Root Systems I. Method of Study with Some Preliminary Results. Annals of botany. 2:159 - 182.

FAOSTAT. 2011. Food and agriculture organization of the united nations. 2011. Em:; consulta: julio, 2011.

FARONI, C.E.; TRIVELIN, P.C.O. 2006. Quantificação de raízes metabolicamente ativas de cana-de-açúcar. Pesquisa Agropecuaria Brasileira. 41:1007 - 1013.

FARONI, C.E. 2004. Sistema radicular da cana-de-açúcar e identificação de raízes metabolicamente ativas. Em:; consulta: julio, 2011.

HACCKETT, C. 1968. A study of the root system of barley. I effects of nutrition on two varieties. New Phytologist.67:287 - 299. doi: 10.1111/j.1469-8137.1968.tb06384.x.

INMAM-BAMBER, N.G.; SMITH, D.M. 2005. Water relations in sugarcane and response to water deficits. Field Crops Research. 92:185 - 202.

HENDRICKS, J. J., HENDRICK, R. L., WILSON, C. A., MITCHELL, R. J., PECOT, S. D.;GUO, D. 2006. Assessing the patterns and controls of fine root dynamics: an empirical test and methodological review. Journal of Ecology. 94:40 - 57. doi: 10.1111/j.1365-2745.2005.01067.x.

KÖPKE, V. 1981. Methods for studyng root growth. 303 - 318p. En: SYMPOSIUM ON THE SOIL/ROOT SYSTEM, 1, Londrina, 1980.

Proceedings. Londrina, Fundação Instituto Agronômico do Paraná.

LEVILLAIN, J.; M'BOU, A. T.; DELEPORTE, P.; SAINT-ANDRÉ, L.; JOURDAN, C. 2011. Is the simple auger coring method reliable for below-ground standing biomass estimation in Eucalyptus forest plantations?. Annal of botany. 108:221 - 230. doi: 10.1093/aob/mcr102.

OTTO, R.; TRIVELIN, P. C. O.; FRANCO, H. C. J.; FARONI, C. E.; VITTI, A. C. 2009. Root system distribution of sugar cane as related to nitrogen fertilization, evaluated by two methods: monolith and probes. Revista Brasileira de Ciência do Solo. 33:601 - 611,

SAINT-ANDRÉ, L.; M’BOU, A. T.; MABIALA, A.; MOUVONDY, W.; JOURDAN, C.; ROUPSARD, O.; NOUVELLON, Y. 2005. Age-related equations for above- and below-ground biomass of a Eucalyptus hybrid in Congo. Forest Ecology and Management. 205:199 - 214. doi: 10.1016/j.foreco.2004.10.006.

SANTOS, H.G.; JACOMINE, P.K.T.; ANJOS, L.H.C.; OLIVEIRA, V.A.; LUMBRERAS, J.F.; COELHO, M.R.; ALMEIDA, J.A.; CUNHA, T.J.F.; OLIVEIRA, J.B. Sistema brasileiro de classificação de solos. Rev. e Ampl. Brasília: Embrapa. 353 p.

SMIT, A. L.; GEORGE, E.;GROENWOLD, J. 2000. Root observations and measurements at (transparent) interfaces with soil. 235 – 271P. En: SMIT A.L.; BENGOUGH A.G.; ENGELS, C.; VAN NOORDWIJK, M.; PELLERIN, S.; VAN DE GEIJN S.C.; EDS. Root Methods: A Hand Book. SpringerBerlin Heidelberg, Berlin. 581 p.

VASCONCELOS, A. C. M.; CASAGRANDE, A. A.; PERECIN, D.; JORGE, L. A. C.; LANDELL, M. G. A. 2003. Avaliação do sistema radicular de cana-de-açúcar por diferentes métodos. Revista Brasileira de Ciência do Solo. 27:849 - 858.

VASCONCELOS, A. C. M.; GARCIA, J. C. 2005. Desenvolvimento radicular da cana-de-açúcar. Cana-de-açúcar: Ambientes de produção. Informações Agronômicas. 110:1 - 5.

How to Cite
Silva-Olaya, A., Pellegrino Cerri, C., & Cerri, C. (2017). Comparison of sampling methods to evaluate sugarcane root system. Revista De Ciencias Agrícolas, 34(1), 7-16.
Research and scientific and technological innovation article