Antimicrobial and molecular resistance profiles of bacterial isolates from poultry production environment

Marina Mota Fernandes; Endria Virgínia Alves  Silva; Beatriz Barbosa Reis; Gessyk Monteiro  Marques; Tullio Teixeira  Deusdará; Hélio de Sousa Brito; Marcela Oliveira  Ferreira; Keytte Fernanda Vieira  Silva; Ana Angelita Sampaio Baptista; Vladimir Pinheiro do Nascimento; Alex Sander Rodrigues  Cangussu

doi:10.22267/rcia.20244103.240

Authors

Marina Mota Fernandes Universidade Federal do Tocantins https://orcid.org/0000-0002-7937-590X
Endria Virgínia Alves Silva Universidade Federal do Tocantins https://orcid.org/0009-0000-1011-1095
Beatriz Barbosa Reis Universidade Federal do Tocantins https://orcid.org/0009-0006-6864-1420
Gessyk Monteiro Marques Universidade Federal do Tocantins https://orcid.org/0009-0003-8174-2320
Tullio Teixeira Deusdará Universidade Federal do Tocantins https://orcid.org/0000-0002-7566-0631
Hélio de Sousa Brito Universidade Federal do Tocantins https://orcid.org/0000-0002-6785-0267
Marcela Oliveira Ferreira Universidade Federal do Tocantins https://orcid.org/0009-0002-1603-0776
Keytte Fernanda Vieira Silva Universidade Federal do Tocantins
Ana Angelita Sampaio Baptista Universidade Estadual de Londrina https://orcid.org/0000-0003-0699-7246
Vladimir Pinheiro do Nascimento Universidade Federal do Rio Grande do Sul https://orcid.org/0000-0002-7720-3274
Alex Sander Rodrigues Cangussu Universidade Federal do Tocantins https://orcid.org/0000-0003-2664-8027

DOI:

https://doi.org/10.22267/rcia.20244103.240

Keywords:

animal health, food safety, multidrug-resistant, poultry farming, strains, 16S rRNA

Abstract

Poultry farming is a relevant industry in Brazil, providing essential animal protein. However, such commercial activity faces significant challenges from bacterial-based diseases, which can impact both animal health and quality product. In this context, this study aimed to identify bacterial strains and evaluate their antimicrobial resistance profiles in poultry farms in southern Tocantins, Brazil. Samples were collected from different stages of poultry production, and the antimicrobial susceptibilities were assessed through antibiogram tests, where bacterial isolates were exposed to 17 antibiotics commonly used in bacterial infection treatment. Bacterial isolates were identified by 16S rRNA gene sequencing, and phylogenetic analysis was used to group them based on genetic similarities. Five isolates were identified: Glutamicibacter creatinolyticus (IGA1), Enterococcus gallinarum (IGA2), Enterobacter mori (IGA3), Lysinibacillus fusiformis (IGA4), and Enterococcus faecalis (IGA5). Antibiotic susceptibility tests revealed significant variations in resistance profiles, with some isolates exhibiting multidrug-resistant (MDR) phenotypes. IGA1 was classified as multidrug-resistant, showing resistance to imipenem, meropenem, ceftazidime, trimethoprim, and doxycycline. IGA2 exhibited resistance to β-lactam antibiotics, including ceftazidime, cefepime, doxycycline, imipenem, meropenem, piperacillin-tazobactam, and tetracycline. IGA3 was resistant to aztreonam and trimethoprim, while IGA4 showed resistance to ceftazidime and cefepime but sensitivity to ciprofloxacin and linezolid. IGA5 did not show resistance to any of the tested antimicrobials. Collectively, our findings highlight bacterial diversity and antibiotic resistance in poultry fams, reinforcing the need for continuous monitoring and effective antimicrobial control strategies to promote animal health, ensure food safety, and prevent the transmission of multidrug-resistant bacteria to humans.

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Antimicrobial and molecular resistance profiles of bacterial isolates from poultry production environment

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