Molecular characterization of Fusarium spp. associated vascular wilt in passion fruit (Passiflora ligularis JUSS)

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DOI:

https://doi.org/10.22267/rcia.223902.180

Keywords:

Fusarium oxysporum, morfología, ITS, TEF 1α, phylogeny vascular wilt, Ilyonectria robusta

Abstract

In Colombia, vascular rotting of passion fruit (P. ligularis) caused by the fungus Fusarium is considered the most important pathological problem in the cultivation of this fruit. Plants affected by the pathogen wilt and die, causing considerable yield losses and increased production costs. The objective of this work was to determine the genetic variability of the Fusarium species associated with this disease. For this, 50 samples were collected from producing farms in three municipalities of the department of Nariño. A total of 35 isolates were characterized morphologically and molecularly. The morphological identification was carried out using taxonomic keys. Molecular characterization was performed by PCR with primers ITS1 – ITS4 and TEF1α. The PCR products were sent to Macrogen (Korea) for sequencing. Sequencing results indicated that Fusarium oxysporum f.sp. passiflorae is associated as a causal agent of the disease. In addition, the presence of Ilyonectria robusta was determined, which, has not been reported before in passion fruit plants. The phylogenetic analysis allowed determine that there was little variability among the isolates evaluated; no grouping was observed associated to the geographical origin of the samples. The morphological characters corroborated the species information obtained from the molecular analysis.

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References

Alastruey-Izquierdo, A.; Cuenca, M.; Monzón, A.; Mellado, E. (2008). Antifungal susceptibility profile of clinical Fusarium spp. isolates identified by molecular methods. J. Antimicrob. Chemother. 61:805–809. doi: 10.1093/jac/dkn022

Aoki, T.; Ward, T.; Kistler, H.; O’Donnell, K. (2012). Systematics, phylogeny and trichothecene mycotoxin potential of Fusarium head blight cereal pathogens. Mycotoxins. 62: 91–102. doi: 10.2520/myco.62.91

Balajee, S.; Borman, A.; Brandt, M.; Cano, J.; Cuenca-Estrella, M.; Dannaoui, E. (2009). Sequence-based identification of Aspergillus, Fusarium, and Mucorales species in the clinical mycology laboratory: where are we and where should we go from here. J. Clin. Microbiol. 47(4):877-84. doi: 10.1128/JCM.01685-08

Caetano, B.; Selvaraj, J.; Marmolejo, D.; Muñoz, J.; Guzmán, A. (2015). Una Metodología No Invasiva Para La Extracción De ADN De Coryphaena Hippurus. Boletín de Investigaciones Marinas y Costeras – INVEMAR. 44(2): 395-399. doi: doi.org/10.25268/bimc.invemar.2015.44.2.33

Chaverri, P.; Salgado, C.; Hirooka, Y.; Rossman, A.; Samuels, G. (2011). Delimitation of Neonectria and Cylindrocarpon (Nectriaceae, Hypocreales, Ascomycota) and related genera with Cylindrocarpon- like anamorphs P. Studies in Mycology. 68: 57-78. doi: doi.org/10.3114/sim.2011.68.03

Daboussi, M.; Langin, T. (1994). Transposable elements in the fungal plant pathogen Fusarium oxysporum. Genética. 93(1-3): 49-59. doi: https://doi.org/10.1007/BF01435239

Dubey, J.; Rajendran, C.; Ferreira, L.; Kwok, O.; Sinnett, D.; Majumdar, D. (2010). A new atypical highly mouse virulent Toxoplasma gondii genotype isolated from a wild black bear in Alaska. J. Parasitol. 96: 13 - 716. doi: 10.1645/GE-2429.1

García, J.; Chamorro, L.; Floriano, J.; Vera, L.; Segura, J. (2007). Enfermedades y plagas del cultivo de la passion fruit (Passiflora ligularis) en el departamento del Huila. Recovered from http://hdl.handle.net/20.500.12324/12713

Geiser, D.; Aoki, T.; Bacon, C. W.; Baker, S. E.; Bhattacharyya, M. K.; Brandt, M. E.; Brown, D. W.; Burgess, L. W.; Chulze, S.; Coleman, J. J.; Correll, J. C.; Covert, S. F.; Crous, P. W.; Cuomo, C. A.; Sybren-De Hoog, G.; Di Pietro, A.; Elmer, W.H.; Epstein, L.; Frandsen, J. N.; Ning, T. (2013). One fungus, one name: defining the genus Fusarium in a scientifically robust way that preserves longstanding use. Phytopathol. 103(5): 400-408. doi:10.1094/PHYTO-07-12-0150-LE

Gordon, T. R. (2017). Fusarium oxysporum and the Fusarium wilt syndrome. Annual review of phytopathology. 55: 23-39. doi: doi.org/10.1146/annurev-phyto-080615-095919

Henao, E.; Hernández, C.; Salazar, C.; Velasco, M.; Gómez, E. (2017). Identificación molecular de aislamientos de Fusarium asociados a maracuyá, en el Valle del Cauca, Colombia. Agron. Mesoam. 29(1):53-61. doi:10.15517/ma.v29i1.27114

Iturralde, P. (2017). Estandarización de una metodología de inoculación de Ilyonectria sp, agente causal de la marchitez en plantas de mora de castilla (Rubus glaucus). Recovered from https://repositorio.espe.edu.ec/handle/21000/12909

Jacobson, D.; Gordon, T. (1991). Fusarium oxysporum f. sp. melonis. A case study of diversity within a forma specialis. Phytopathology. 81: 1064-1067.

Londoño, J. (2012). Evaluación de la resistencia genética de especies de Passiflora spp a Fusarium spp, agente causal de la “secadera”. Recovered from https://repositorio.unal.edu.co/handle/unal/11131

Leslie, F.; Summerell, B. (2006). The Fusarium laboratory manual. Manhattan, United States of America: Published, blackwell. 387p.

Lin, C.; Koval, A.; Tishchenko, S.; Gabdulkhakov, A.; Tin, U.; Solis, G. P.; Katanaev, V. L. (2014). Double Suppression of the Gα Protein Activity by RGS Proteins. Mol. Cell. 53(4):663-671. doi: https://doi.org/10.1016/j.molcel.2014.01.014

Landlinger, C.; Bašková, L.; Preuner, S.; Willinger, B.; Buchta, V.; Lion, T. (2009). Identification of fungal species by fragment length analysis of the internally transcribed spacer 2 region. Eur. J. Clin. Microbiol Infect. Dis. 28(6): 613-622. doi: doi.org/10.1007/s10096-008-0683-3

MADR - Ministerio de Agricultura y Desarrollo rural de Colombia. (2022). Análisis, estadísticas, passion fruit. Recovered from https://www.agronet.gov.co/estadistica/Paginas/home.aspx?cod=1

Melgarejo, L.; Rodríguez-Castillo, N. (2015). Granadilla Passion fruit (Passiflora ligularis Juss): Caracterización ecofisiológica del cultivo. Bogotá: Universidad Nacional de Colombia. 304 p.

McDonald, F.; Porras, V.; Sánchez, J.; Mignucci, J.; Torres, L.; Hepperly, P. R.; Clarke, A. (1992). Diseases of economic importance in citrus (grapefruit) and avocado. Recovered from http://www.sidalc.net/cgibin/wxis.exe/?IsisScript=iicacr.xis&method=post&formato=2&cantidad=1&expresion=mfn=018605

National Library of Medicine. (2021). BLAST - Basic Local Alignment Search Tool. Recovered from https://blast.ncbi.nlm.nih.gov/Blast.cgi

Nalim, F. A.; Elmer, W. H.; McGovern, R. J.; Geiser, D. M. (2009). Multilocus phylogenetic diversity of Fusarium avenaceum pathogenic on lisianthus. Phytopathology. 99:462-468. doi: https://doi.org/10.1094/PHYTO-99-4-0462

Nelson, P.; Toussoun, T.; Marasas, W. (1983). Fusarium species: an illustrated manual for identification. The Pennsylvania State: University Press, University park. 30p.

Osorio, J.; Martínez, E.; Hio, J.; Aguirre, J.; Vergara, J.; Luque, N.; Rojas, E.; Cruz, G. N. (2020). Caracterización sanitaria de los cultivos de granadilla, gulupa y maracuyá en Colombia, con especial referencia a la secadera causada por por Fusarium solani f. sp. Passiflorae. Primera Ed, Vol. 1. Mosquera, Colombia: Agrosavia. Hilos Tensados. doi: https://doi.org/10.21930/agrosavia.investigation.7403381

Ortiz, H. (2012). Etiología de enfermedades asociadas a fusariosis en el cultivo de gulupa (Passiflora edulis Sims.) en la región del Sumapaz. Recovered from https://repositorio.unal.edu.co/handle/unal/9567

O’Donnell, K.; Kistler, H.; Cigelnik, E.; Ploetz, R. (1998). Multiple evolutionary origins of the fungus causing panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Rev. Proc. Nal. Acad. Sci. 95:2044-2049

Pathrose, B.; Jones, E. E.; Jaspers, M. V.; Ridgway, H. J. (2010). Development of a grapevine detached root assay for assessing pathogenicity of Cylindrocarpon spp. New Zealand Plant Protection. 63: 24–7.

Patiño, M.; Pérez, O.; Arbeláez, G. (2015). Diagnóstico del agente causal de la “Secadera” en passion fruit (Passiflora ligularis Juss) en Colombia. Recovered from http://rmf.smf.org.mx/suplemento/docs/volumen332015/resumenesposters.pdf.

Retana, K.; Ramírez-Coché, J.; Castro, O.; Blanco-Meneses, M. (2017). Caracterización morfológica y molecular de Fusarium oxysporum f. sp. apii asociado a la marchitez del apio en Costa Rica. Agronomia Costarricence. 42(1): 115-126. doi: http://dx.doi.org/10.15517/rac.v42i1.32199

Rodríguez, H. (2013). Caracterización de aislamientos de Fusarium spp. Obtenidos de zonas productoras de uchuva (Physalis peruviana) en Cundinamarca y Boyacá. Recovered from https://repositorio.unal.edu.co/bitstream/handle/unal/50425/07790760.2013.pdf?sequence=1&isAllowed=y

Salazar, C. (2016). Caracterización molecular y morfológica de aislamientos de Fusarium spp. productores de micotoxinas. Retrieved from https://repositorio.unal.edu.co/handle/unal/57485

Salazar, C.; Lagos, L. E.; Díaz, V.; Mora, S.; Betancourth, C. (2020). Caracterización de Fusarium spp. asociado con la pudrición basal de la cebolla de rama. Rev. U.D.C.A Act. & Div. Cient. 23(1): e1471. doi: doi. org/10.31910/rudca.v23.n1.2020.1471

Sampietro, D.; Marín, P.; Iglesias, J.; Presello, D.; Vattuone, M.; Catalan, C. (2010). A molecular based strategy for rapid diagnosis of toxigenic Fusarium species associated to cereal grains from Argentina. Fungal. Biol. 114(1): 74-81. doi: https://doi.org/10.1016/j.mycres.2009.10.008

Silva, A. S.; Oliveira, E. J.; Haddad, F.; Laranjeira, F. F.; Jesus, O. N.; Oliveira, S. A. S.; Costa, M. A. P. C.; Freitas, P. X. (2013). Identification of passion fruit genotypes resistant to Fusarium oxysporum f. sp.passiflorae. Tropical Plant Pathology, Brasília. 38(3): 236-242. doi: https://doi.org/10.1590/S1982-56762013005000008

Tamayo, P. (1999). Estudio para el control de la secadera (Nectria haematococca Berk. & Br.) de la passion fruit (Passiflora ligularis Juss.): Evaluación de patrones existentes y prácticas de manejo integrado: informe técnico. Recovered de http://agris.fao.org/agris-search/search.do?recordid=co20030009164

Tamayo, P. (2001). Meloidogyne incógnita en passion fruit. Recovered from https://www.cabdirect.org/cabdirect/abstract/20013115577

The online auction Color Chart. (2004). The new language of color for buyers and sellers. Palo alto, calif: Online aduction color chart co. 20p.

Turner, A. S.; Lees, A. K.; Rezanoor, H. N.; Nicholson, P. (1998). Refinement of PCR-detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum. Plant Pathol. 47: 278-288. doi: https://doi.org/10.1046/j.1365-3059.1998.00250.x

White, T.; Bruns, T.; Lee, S.; Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A.; Gelfand, D. H.; Sninsky, J. J.; White T.J. PCR Protocols.: A Guide to Methods and Applications. pp. 315-322. New York: Academic Press, Inc.

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Published

2022-06-01

How to Cite

Salazar-Gonzalez, C., Yela-Caicedo, O., & Gomez-Espinoza, B. (2022). Molecular characterization of Fusarium spp. associated vascular wilt in passion fruit (Passiflora ligularis JUSS). Revista De Ciencias Agrícolas, 39(2), 33–46. https://doi.org/10.22267/rcia.223902.180