Bio regulation of Meloidogyne incognita (Kofoid & White) Chitwood and M. javanica (Treub) Chitwood complex in coffee roots

Authors

DOI:

https://doi.org/10.22267/rcia.223902.189

Keywords:

microorganisms, prevention, protection, rhizosphere, root-knot nematodes, sustainability

Abstract

The Meloidogyne incognita and M. javanica complex cause damage to the coffee plants’ roots with loss of productive potential. Traditional management against this complex is based on the use of chemical molecules which causes problems in soil health and harmful to the environment. In this regard, the Micosplag® biotechnological input [Paecilomyces lilacinus (Thom) Samson, 1974, Metarhizium anisopliae (Metsch) Sorokin and Beauveria bassiana (Balsamo) Vuillemin] and Tricho-D® (Trichoderma harzianum Rifai, 1969) were evaluated under greenhouse conditions against root-knot nematode complex. A dose of 2g. L-1 of water was used for Micosplag® and 10g. L-1 of water for Tricho-D®, applying a volume of 20mL.1 per plant. The nematode inoculation was carried out eight days after the sowing, using 2500 eggs per plant. Bioinputs were applied preventively and also as bioregulators of an existing nematodes population. Nine treatments, which corresponded to different moments of application of bioinputs in soil and soil plus vermocompost were evaluated. Results showed the lowest levels of infection when the Micosplag® bioinput was applied preventively (infection level of 6%), followed by the Tricho-D® treatment (infection 12%). There were statistical differences with the controls which were inoculated alone with the nematodes. When the two Bioinputs were applied preventively, they offered greater protection to the roots against nematodes. Dry weights of root and the aerial part of the plants showed the lowest values in treatments where the nematode was inoculated. Six months after the experiment was established, the three fungi that compose the Micosplag® Bioinput were isolated from the rhizosphere of the coffee plants.

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References

Affokpon, A.; Coyne, D.L.; Htay, Ch.Ch.; Agbede R.D.; Lawouin L.; Coosemans J. (2011). Biocontrol potential of native Trichoderma isolates against root-knot nematodes in West African vegetable production systems. Soil Biology & Biochemistry. 43(3): 600-608. doi: https://doi.org/10.1016/j.soilbio.2010.11.029

Ahmed, H.M.; Gad, S.B.; El-Sherif, A.G.; El-Hadidy, E.M. (2022). Efficacy of Five Biopesticides for the Management of Root-Knot Nematode, Meloidogyne incognita Infecting Pepper (Capsicum annum L.) Plants. Egyptian Journal of Agronematology. 21(1): 23-33. doi: https://10.21608/EJAJ.2022.218707

Ali, T.; Mubeen, M.; Jamil, Y.; Ahmad, U.; Khan, A.H., Aiman Iqbal, S.; Baber, Y.; Hassan, F.; Usman, M.H.; SohaiL, A.M.; Abbas, A. (2021). An overview of root-knot nematodes and their management. Journal of Entomology and Zoology Studies. 9(1): 35-40. Recuperada de www.entomoljournal.com

Anastasiadis, I.A.; Giannakou, I.O.; Prophetou-Athanasiadou, D.A.; Gowen S.R. (2008). The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodes. Crop Protection. 27(3-5): 352-361. doi: https://doi.org/10.1016/j.cropro.2007.06.008

Arango, B.L.G. (1977). Estudio del proceso infectivo y la histopatología del complejo de nemátodos Meloidogyne incognita-M. javanica sobre plantas de cafeto (No. Doc. 1593)*. Santafé de Bogotá: CO-BAC.

Bendezu, C.R.E. (2017). Control de Meloidogyne sp. en vivero de Coffea arabica L. mediante quinoleína fenólica, Paecilomyces lilacinus y estiércol en la zona de Satipo. Recuperada de http://hdl.handle.net/20.500.12894/4026

Cardona, B.N.L.; Leguizamón, J.E. (1997). Aislamiento y patogenicidad de hongos y bacterias al nematodo del nudo radical del café Meloidogyne spp. Goeldi. Fitopatología Colombiana (Colombia). 21(1): 39-52.

Cepeda, S.M.; Gallegos, M.G. (2004). Evaluación de la efectividad biológica, de biostat Paecilomyces lilacinus (Thom) Samsom, para el control de nematodos en papa (Solanum tuberosum L.) en Navidad, Galeana, Nuevo, León, México. Revista Agraria. 1(3): 349-359.

Collange, B.; Navarrete, M.; Peyre, G.; Mateille, T.; Tchamitchian, M. (2011). Root-Knot nematode (Meloidogyne) management in vegetables crop productions: The challenge of an agronomic system analysis. Crop Protection. 30(10): 1-12. doi: https://doi.org/10.1016/j.cropro.2011.04.016

Giraldo, F.M.A.; Leguizamón, C.J.E.; Chaves, C.B. (1998). Evaluación de Paecilomyces lilacinus (Thom.) Samson para el control de Meloidogyne sp. Goeldi. En almácigos de café (Coffea arabica L.) Variedad Caturra. Fitopatología Colombiana. 21(2): 104-117.

Gutiérrez, C.E. (2020). Microorganismos antagonistas para el manejo de Meloidogyne spp. en el cultivo de café en el distrito de San Martín de Pangoa. Perú. Recuperada de http://hdl.handle.net/20.500.12894/6455

Hashem, M.; Abo-Elyousr, K.A. (2011). Management of the root-knot nematode Meloidogyne incognita on tomato with combinations of different biocontrol organisms. Crop Protection. 30(3): 285-292.

doi: https://doi.org/10.1016/j.cropro.2010.12.009

Hincapie, R.D.; Leguizamón, C.J.E. (1999). Efecto de Verticillium chlamydosporium en el control de Meloidogyne spp. En almácigos de café, Var. Caturra. Cenicafé. 50(4): 286-298.

Hussey, P.S.; Barker, K.R. (1973). A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter. 57: 1025-1028.

Karabörklü, S.; Aydınlı, V.; Dura, O. (2022). The potential of Beauveria bassiana and Metarhizium anisopliae in controlling the root-knot nematode Meloidogyne incognita in tomato and cucumber. Revista de entomología de Asia y el Pacífico. 25(1): 2-6. doi: https://doi.org/10.1016/j.aspen.2021.101846

Kumar, K.K.; Arthurs, S. (2021). A. Recent advances in the biological control of citrus nematodes: A review. Biological Control. 157: 1-8.

doi: https://doi.org/10.1016/j.biocontrol.2021.104593

Leguizamón, C.J.E. (1976). Relación entre poblaciones de Meloidogyne spp en el suelo y daño causado en cafetales establecidos. Cenicafé. 27(4):174-184.

Leguizamón, C.J.E.; Padilla, H.B.E. (2001). Efecto de Beauveria bassiana y Metarhizium anisopliae en el control del nematodo del nudo radical del café. Cenicafé. 52(1): 29-41. Recuperada de http://hdl.handle.net/10778/767

Mendoza, G. (2021). Manejo agronómico de plagas que afectan el sistema radical del viñedo. Ediciones INTA. Argentina: Estación Experimental Agropecuaria de Mendoza, INTA. 33 p.

Recuperada de http://hdl.handle.net/20.500.12123/10025

Monjil, M.S.; Ahmed, S. (2017). Paecilomyces lilacinus on egg hatching and larval population of Meloidogyne sp. Bangladesh Journal Plant Pathology. 33(1-2): 71-78.

Moreno, G.A.; Huertas, V.; Diánez, F.; Sánchez, M.B.; Santos, M. (2020). Paecilomyces and its Importance in the Biological Control of Agricultural Pests and Diseases. Plants. 9(12): 1746. doi: https://doi.org/10.3390/plants9121746

Puma, Q.E.E. (2021). Caracterización del nematodo del nódulo de la raíz (Meloidogyne spp.) en el cultivo de café (Coffea arabica L.) en San Juan del Oro – Sandía, Puno. Recuperada de http://repositorio.unap.edu.pe/handle/UNAP/15373

Quesada, M.Y.; Fernández, G.E.; Casanueva, M.K.; Ponce, G.E.; Márquez, G. M.E. (2019). Actividad biológica de nuevas cepas cubanas de Trichoderma spp. efectivas en el control de Meloidogyne incognita (Kofoid & White) Chitwood. Revista Cubana de Ciencias Biológicas. 7(1): 1-9.

Rivillas, O.C.A. (2003). Nematodos. In: Gil, V.L.F.; Castro, C.B.L.; Cadena, G.G. Enfermedades del café en Colombia. Chinchiná (Colombia). Cenicafé. 224p. Recuperada de http://hdl.handle.net/10778/993

Rumbos, CH.; Reimann, S.; Kiewnick, S.; Sikora, R.A. (2006). Interactions of Paecilomyces lilacinus strain 251 with the mycorrhizal fungus Glomus intraradices: Implications for Meloidogyne incognita control on tomato. Biocontrol Science and Technology. 16(9): 981-986.

doi: https://doi.org/10.1080/09583150600937667

Sahebani, N.; Hadavi, N. (2008). Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Soil Biology Biochemistry (Inglaterra). 40(8):2016-2020. doi: https://doi.org/10.1016/j.soilbio.2008.03.011

Siddiqui, Z.A.; Mahmood, I. (1996). Biological control of plant parasitic nematodes by fungi: A review. Bioresource Technology. 58: 229-239.

doi: https://doi.org/10.1016/S0960-8524(96)00122-8

Stefanova, N.M. (2007). Introducción y eficacia técnica del biocontrol de fitopatógenos con Trichoderma spp. en Cuba. Fitosanidad. 11(3):75-79. Recuperada de https://www.redalyc.org/articulo.oa?id=209116023011

Youssef, M.M.A.; El-Nagdi, W.M.A.; Lotfy, D.E.M. (2020). Evaluation of the fungal activity of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus as biocontrol agents against root-knot nematode, Meloidogyne incognita on Cowpea. Bulletin of the National Research Centre. 44(112): 1-11.

doi: https://doi.org/10.1186/s42269-020-00367-z

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Published

2022-12-27 — Updated on 2023-01-24

How to Cite

Castro-Toro, A. M., & Rivillas-Osorio, C. A. (2023). Bio regulation of Meloidogyne incognita (Kofoid & White) Chitwood and M. javanica (Treub) Chitwood complex in coffee roots. Revista De Ciencias Agrícolas, 39(2), 128–142. https://doi.org/10.22267/rcia.223902.189