contadores web
Skip to main navigation menu Skip to main content Skip to site footer
##common.pageHeaderLogo.altText##

Scientific and technological research article

Vol. 17 No. 1 (2015)

Antimicrobial resistance of bacteria isolated from veterinary clinics in the city of Ibague, Colombia

Submitted
November 18, 2015
Published
2015-05-26

Abstract

Objective: To isolate bacteria circulating in veterinary clinics in the city of Ibague for knowing its antimicrobial resistance profile and in some cases, its ability to transfer this resistance to susceptible bacteria. Materials and Methods: Samples of 10 clinics that underwent bacterial culture, biochemical identification, antimicrobial susceptibility testing and bacterial conjugation to transfer this resistance were taken. The methodological design was quasi-experimental and the analysis of the results was made using descriptive statistics. Results: In all areas of the 10 clinical multiresistant potentially pathogenic bacteria which belonged to 8 of 16 species isolated were found. The microorganisms that occurred more frequently in different clinical places were: Staphylococcus intermedius, Acinetobacter baumannii, Pantoea agglomerans, Klebsiella pneumoniae and Burkhordelia cepacia. The places where multiresistant microorganisms were most frequently isolated were the outpatients’ floor and the clinical examination table. The resistance occurred mainly to amoxicillin and chloramphenicol. The study shows the presence of potential pathogens causing nosocomial infections, which constitute a reservoir of resistance genes to antibiotics for non-resistant pathogenic bacteria.

References

  1. FAO; OIE; OMS. Comunicado No 11/317. Boletín ONU, Sede de la Reunión técnica de alto nivel sobre enfermedades zoonóticas. México, Cuba, República Dominicana: Centro de Información de las Naciones Unidas; 2011.
  2. Andrews B. Antimicrobial use in animal husbandry and its relationship to resistant bacteria in human health. Paris. In OIE International standards on antimicrobial resistance. Paris: 2013.
  3. Diaz F. Antimicrobial use in animals: analysis of the OIE survey on monitoring of the quantities of antimicrobial agents used in health. Paris. In OIE International standards on antimicrobial resistance. Paris: 2013
  4. Scott RD. The direct medical costs of health care-associated infections in U.S. hospitals and the benefits of prevention. National center for preparedness, detection, and control of infectious diseases. 2009.
  5. Comité Internacional de la OIE. Resolución XXX. Directrices para el control de la resistencia a los antimicrobianos. París: 2003.
  6. Justine A, Johnson D. Nosocomial infections. The veterinary clinics small animal practice. 2002; 32:1101-1126.
  7. Benedict KM, Morley PS, Van Metre DC. Characteristics of biosecurity and infection control programs at veterinary teaching hospitals. Journal of the American Veterinary Medical Association. 2008;233(5): 767- 773.
  8. Zordan S, Prenger-Berninghoff E, Weis R, Van der Reijden T, Van den Broek P, Baljer G, Dijkshoorn Lenie. Multidrug-resistant Acinetobacter baumannii in veterinary clinics, Germany. Emerg Infect Dis. 2011;17(9):1751-4.
  9. Haenni M, Ponsin C, Metayer V, Medaille C, Madec J. Veterinary hospital-acquired infections in pets with a ciprofloxacin-resistant CTX-M-15-producing Klebsiella pneumoniae ST15 clone. J Antimicrob Chemother. 2012;67(3):770-1.
  10. Weese JS, Faires M, Rousseau J, Bersenas AM, Mathews KA. Cluster of methicillin-resistant Staphylococcus aureus colonization in a small animal intensive care unit. J Am Vet Med Assoc. 2007;231(9):1361-4.
  11. Julian T, Singh A, Rousseau J, Weese JS. Methicillin-resistant staphylococcal contamination of cellular phones of personnel in a veterinary teaching hospital. BMC Research Notes 2012,5:193.
  12. Murphy C, Reid-Smith RJ, Boerlin P, Weese JS, Prescott JF, Janecko N, Hassard L, McEwen SA. Escherichia coli and selected veterinary and zoonotic pathogens isolated from environmental sites in companion animal veterinary hospitals in southern Ontario. Can Vet J 2010;51:963–972.
  13. Youn JH, Park YH, Hang’ombe B, Sugimoto C. Prevalence and characterization of Staphylococcus aureus and Staphylococcus pseudintermedius isolated from companion animals and environment in the veterinary teaching hospitalin Zambia, Africa. Comparative Immunology, Microbiology and Infectious Diseases. 2014;37(2):123– 130.
  14. Mackay D. Key success factors for surveys of antimicrobial sales and use in the European Union. O.I.E. Conferencia Mundial de la OIE sobre el uso responsable y prudente de los agentes antimicrobianos en los animales. París:2013.
  15. Donado P, Clavijo V, León M. Prevalence, risk factors, and antimicrobial resistance (AMR) profiles of Salmonella sp. in commercial broiler farms in two important poultry-producing regions of Colombia. JFP. 2011, 75(6):1134-1138.
  16. Vargas J, Máttar S, Monsalve S. Bacterias patógenas con alta resistencia a antibióticos: estudio sobre reservorios bacterianos en animales cautivos en el zoológico de Barranquilla. Infectio. 2010; 14(1):6-19.
  17. Clinical and Laboratory Standars Institute (CLSI). Performance standards for antimicrobial susceptibility testing. Document M100-S18. Eighteenth edition ed.; 2009.
  18. Calderón R, Sacsaquispe R, Pasterán F.G, Galas M.F, Javier Soto J.P y cols. Caracterización molecular de klebsiella pneumoniae y Enterobacter cloacae productoras de ß-lactamasas de espectro extendido tipo shv-5 en una unidad de cuidados intensivos neonatal de lima. Rev peru med exp salud pública 2003;20(3).
  19. Clinical and Laboratory Standards Institute CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. 2012;32(2)
  20. Sasaki T, Kikuchi K, Tanaka Y, Takahashi N, Shinichi Kamata S, and Hiramatsu K. Reclassification of phenotypically identified Staphylococcus intermedius strains. J clinl Microbiol.2007;45(9):2770–2778
  21. DeBoer D. Canine staphylococcal pyoderma’, US Companion Animal Health. 2006:26-28
  22. Sanz L, Junco C. Infecciones bacterianas de las heridas operatorias-Identification of the etiology of bacterial infections of surgical wounds. Hospitales Veterinarios. 2009; 1(1).
  23. Jara OA, Avendaño RP, Navarro VC. Identificación y estudio de susceptibilidad antimicrobiana de bacterias potencialmente responsables de infecciones nosocomiales en los hospitales veterinarios de la Universidad de Chile. Av. Cs. Vet. 2009;1 & 2: 11-17.
  24. Howard A, O’Donoghue M, Feeney A, Sleator RD. Acinetobacter baumannii. An emerging opportunistic pathogen. Virulence. 2012;3(3):243-250.
  25. Lemos EV, De la Hoz Restrepo F, Alvis N, Quevedo E, Cañon O, León Y. Mortalidad por Acinetobacter baumannii en unidades de cuidados intensivos en Colombia. Rev Panam Salud Publica. 2011;30(4): 287– 94.
  26. Gil DM Bacteremia de curso fatal por Burkholderia cepacia: Revisión de la literatura a propósito de un caso clínico. Rev Chil Infect. 2001;18(1): 41-44
  27. Sousa SA, Ramos CG, and Letãio JH. Burkholderia cepacia Complex: Emerging multihost pathogens equipped with a wide range of virulence factors and determinants. Int J Microbiol. Volume 2011, Article ID 607575, 9 pages doi:10.1155/2011/607575
  28. Frias A. Burkholderia cepacia ( B. cepacia), a new pathogen in nosocomial infections clinic cases. Enf Inf. Microbiol. 2008; 28(1):19-23.
  29. Collobert C, Fortier G, Perrin R, Letot G, Andrioud D. Bacteria and fungi isolated from equine tracheobronchial aspirates. Prat Vet Equin. 1995;27:91-96.
  30. Akkoç A, Kocabiyik Al, Özyigit MÖ, Cangül IT, Yilmaz R, Özakin c. Burkholderia cepacia and aeromonas hydrophila septicemia in an african grey parrot (psittacus erithacus erithacus). turkish journal of veterinary & animal sciences. 2008;32(3):233-236.
  31. Fariñas MC, Martínez-M L. Infecciones causadas por bacterias gramnegativas multirresistentes: enterobacterias, Pseudomona aeruginosa, Acinetobacter baumannii y otros bacilos. / Enferm Infecc Microbiol Clin. 2013; 31(6):402–409
  32. Chaparro JL, Hernández YK, Castellanos V, Arcila VH. Aislamiento, identificación y antibiograma de las bacterias presentes en el Centro médico quirúrgico veterinario Universidad Cooperativa de Colombia. Rev Col Cienc Pec. 2005;18(4):381-381.
  33. Ocana C, Rocchi A, Gasparotto I, et al. Bacteriemia por enterobacterias en adultos en un hospital universitario: análisis de cinco años. Rev. Argent. Microbiol. 2007;39(1):38-43.
  34. Lupión C, López LE, Rodríguez JB. Medidas de prevención de la transmisión de microorganismos entre pacientes hospitalizados. Higiene de manos. Enferm Infecc Microbiol Clin. 2014; 32(9):603–609.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...