Introduction: A high-quality clinical laboratory plays a key role in healthcare delivery. Staff motivation and training directly influence the quality of service. Objective: To explore how laboratory personnel perceive the factors that influence service quality in clinical laboratories in two municipalities of the colombian pacific region. Materials and Methods: This qualitative study used an ethnographic approach and a constructivist paradigm. Researchers conducted semi-structured interviews with 42 staff members and analyzed the data thematically using Atlas.ti software. Results: Participants identified regulatory knowledge, quality program implementation, ongoing training, and working conditions as key elements that drive performance and sustain quality systems. Conclusions: Strengthening technical skills, regulatory understanding, and employment conditions remains essential. Institutional commitment plays a critical role not only in developing sustainable quality systems but also in fostering a positive organizational climate, increasing motivation, and enhancing job satisfaction, factors that drive continuous improvement in service delivery.